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<p><i>Camelina sativa</i> (L.) Crantz is one of the oil plants cultivated in diverse world regions since ancient times. Recently, this plant has been highly considered due to several reasons, such as resistance to drought stress, diseases, and a short growth period. This study examined the genetic diversity, polymorphism, and population structure in 9 Iranian cultivated populations of <i>Camelina</i> cv. <i>Soheil</i> is using ISSR molecular markers to identify possible genotypes for improving the genetic polymorphism of its populations. Nuclear genomes were extracted using the CTAB protocols and amplified using diverse ISSR primers. Data were analyzed using different software, including PAST ver. 1.89, POPGENE ver. 1.3.1, GenALEx ver. 6.5, and STRUCTURE ver. 2.3.4. We found a limited level of genetic diversity among the populations, which were explored by the low amounts of Gst, polymorphism percentage, and total heterozygosity. However, a large proportion of the significant genetic diversity (62%) belonged to within-population variation. Meanwhile, the estimated amounts of pairwise population differentiation widely differed among the populations, which were supported by the population’s polymorphism amount and the PCA analysis. The evaluated populations were divided into two major clusters in the UPGMA tree that was constructed based on Nei’s genetic distance. STRUCTURE analysis indicated that each identified cluster can be considered as a genotype group. A moderate rate of gene flow (Nm = 0.55) occurred among the populations that were characterized by a relatively low genetic differentiation index (Gst = 0.47). Our findings agreed with results of previous molecular investigations on this economically important plant. Additionally, ISSR markers have enough potential in determining the distribution proportion of genetic diversity within and among populations and populations genetic structure in this species. However, the identified genotypes can be taken into consideration for the co-cultivation of these genotypes together and future breeding programs to produce new lines with a wide range of genetic diversity.</p>

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Identification of genotype groups in Iranian Camelina sativa (L.) Crantz (Brassicaceae) cultivated populations using ISSR molecular markers

  • 期刊论文

  • 发表日期：

    2026年7月3日

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  • Seyed Mehdi Talebi,
  • Zahra Dehbashi,
  • Maryam Keshavarzi,
  • Mansour Ghorbanpour

摘要

Camelina sativa (L.) Crantz is one of the oil plants cultivated in diverse world regions since ancient times. Recently, this plant has been highly considered due to several reasons, such as resistance to drought stress, diseases, and a short growth period. This study examined the genetic diversity, polymorphism, and population structure in 9 Iranian cultivated populations of Camelina cv. Soheil is using ISSR molecular markers to identify possible genotypes for improving the genetic polymorphism of its populations. Nuclear genomes were extracted using the CTAB protocols and amplified using diverse ISSR primers. Data were analyzed using different software, including PAST ver. 1.89, POPGENE ver. 1.3.1, GenALEx ver. 6.5, and STRUCTURE ver. 2.3.4. We found a limited level of genetic diversity among the populations, which were explored by the low amounts of Gst, polymorphism percentage, and total heterozygosity. However, a large proportion of the significant genetic diversity (62%) belonged to within-population variation. Meanwhile, the estimated amounts of pairwise population differentiation widely differed among the populations, which were supported by the population’s polymorphism amount and the PCA analysis. The evaluated populations were divided into two major clusters in the UPGMA tree that was constructed based on Nei’s genetic distance. STRUCTURE analysis indicated that each identified cluster can be considered as a genotype group. A moderate rate of gene flow (Nm = 0.55) occurred among the populations that were characterized by a relatively low genetic differentiation index (Gst = 0.47). Our findings agreed with results of previous molecular investigations on this economically important plant. Additionally, ISSR markers have enough potential in determining the distribution proportion of genetic diversity within and among populations and populations genetic structure in this species. However, the identified genotypes can be taken into consideration for the co-cultivation of these genotypes together and future breeding programs to produce new lines with a wide range of genetic diversity.