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<p>Nicotine contained in tobacco, endangers&#xa0;human health and&#xa0;environment. Nicotinophilic bacteria might serve as sustainable mode of nicotine degradation in tobacco industrial waste. In current study, nicotine degrading bacteria were isolated from soil and&#xa0;analyzed through growth curve analysis and biochemical and molecular characterization. Nicotine degradation potential was confirmed through nicotine removal assay, FTIR and GC–MS analysis. Fifteen enzymes sequences were retrieved from Uniprot database and analyzed via MEME suite and TOMTOM web server, SOPMA tool, Alphafold and AutoDock Vina. Five bacteria were identified as&#xa0;<i>Arthrobacter nitrophenolicus</i>&#xa0;SBBN1,&#xa0;<i>Pseudomonas putida</i>&#xa0;SBBN2,&#xa0;<i>A. oxidans</i>&#xa0;SBBN3,&#xa0;<i>P. aeruginosa</i>&#xa0;SBBN4 and&#xa0;<i>Bacillus cereus</i>&#xa0;SBBN5. <i>A. nitrophenolicus</i>&#xa0;SBBN1showed highest nicotine degradation (66&#xa0;mg/L per 26&#xa0;h). GC–MS analysis identified metabolites of three pathways.&#xa0;i. e., pyridine, pyrrolidine and variant of pyridine and pyrrolidine (VPP) pathways.&#xa0;<i>In-silico</i>&#xa0;characterization revealed the&#xa0;conserved motifs of enzymes&#xa0;as&#xa0;binding sites for various&#xa0;transcription factors. Three levels of secondary (2D) structure. i. e., alpha helix (14–200%), extended strand (27–82%) and random coil (74–245%) were observed. Docking of enzymes with their substrates revealed binding energies (-3.9 to -6.6). Bacteria explored in current study might contribute to sustainable bioremediation of tobacco waste.</p>

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Insights into the nicotine bioremediation potential of bacteria isolated from tobacco field soil

  • 期刊论文

  • 发表日期：

    2026年6月22日

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  • Bisma N. Siddiqui,
  • Fatima Muccee,
  • Amal H. I. Al Haddad,
  • Nadia Hussain,
  • Ali Raza

摘要

Nicotine contained in tobacco, endangers human health and environment. Nicotinophilic bacteria might serve as sustainable mode of nicotine degradation in tobacco industrial waste. In current study, nicotine degrading bacteria were isolated from soil and analyzed through growth curve analysis and biochemical and molecular characterization. Nicotine degradation potential was confirmed through nicotine removal assay, FTIR and GC–MS analysis. Fifteen enzymes sequences were retrieved from Uniprot database and analyzed via MEME suite and TOMTOM web server, SOPMA tool, Alphafold and AutoDock Vina. Five bacteria were identified as Arthrobacter nitrophenolicus SBBN1, Pseudomonas putida SBBN2, A. oxidans SBBN3, P. aeruginosa SBBN4 and Bacillus cereus SBBN5. A. nitrophenolicus SBBN1showed highest nicotine degradation (66 mg/L per 26 h). GC–MS analysis identified metabolites of three pathways. i. e., pyridine, pyrrolidine and variant of pyridine and pyrrolidine (VPP) pathways. In-silico characterization revealed the conserved motifs of enzymes as binding sites for various transcription factors. Three levels of secondary (2D) structure. i. e., alpha helix (14–200%), extended strand (27–82%) and random coil (74–245%) were observed. Docking of enzymes with their substrates revealed binding energies (-3.9 to -6.6). Bacteria explored in current study might contribute to sustainable bioremediation of tobacco waste.