<p>Recent genetic studies on the genus <i>Pelophylax</i> have explored regional distribution patterns and evolutionary relationships, helping to clarify taxonomic uncertainties and support the monitoring of biological invasions. Within this group, <i>Pelophylax kurtmuelleri</i> has received particular attention in Europe due to its invasive potential. Despite earlier reports of its occurrence, it has not been regarded as a native component of the Croatian fauna. Here, we confirm the presence of <i>P. kurtmuelleri</i> in Croatia and provide evidence that it is native to southern Dalmatia, specifically the Konavle Field and the Neretva River Delta. Analyses of one nuclear (SAI-1) and two mitochondrial markers (COI and ND2) identified 34 individuals of <i>P. kurtmuelleri</i>, with no evidence of other <i>Pelophylax</i> species, hybrids, or hybridogenetic complexes. These findings complement previous studies based solely on mitochondrial DNA, which suggested the presence of <i>P. kurtmuelleri</i> across the Adriatic basin, including Croatia and adjacent regions. Given the morphological similarity between <i>P. kurtmuelleri</i> and <i>Pelophylax ridibundus</i>, as well as the frequent occurrence of hybridization within the genus, our results highlight the importance of combining nuclear and mitochondrial markers to resolve taxonomic ambiguity. This integrative approach provides clear evidence for the existence of pure <i>P. kurtmuelleri</i> populations in southern Croatia, supporting its status as a native species in this region.</p>