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<p><i>Cordyceps militaris,</i> an entomopathogenic fungus is known for its numerous bioactive metabolites, particularly cordycepin. The biosynthetic pathway involved in cordycepin production is still elusive. There is a need to identify the existence of cordycepin biosynthesis genes in other ascomycetes fungal genera. This study explores this possibility through the evaluation of the occurrence of orthologs of <i>cns1-3</i> genes which are essential for cordycepin production in <i>Cordyceps militaris.</i> The <i>cns1-3</i> genomic and protein sequences of <i>Cordyceps militaris</i> were utilized to perform pBLAST and motif analysis, to identify ortholog genes in other ascomycetes fungi. These proteins were subjected to physiochemical characterization and their molecular functions were predicted. The multiple sequence alignment studies revealed variations as well as similarities in conserved domains of <i>Cordyceps militaris</i> and its orthologs. The protein motif studies further helped in prediction of the structural similarities for the secondary and tertiary structures. The results underline the cryptic nature of these secondary metabolite genes by demonstrating the existence of putative cordycepin synthesis proteins in other fungi. In vivo research studies, such as heterologous gene expression analyses can be beneficial to complete comprehension of the potential for cordycepin production in these fungi. These orthologs could be the putative candidates for verifying the new methods of gene elicitation for improved synthesis of bioactive metabolites. This study provides a valuable insight for diversification of cordycepin bio-synthesis pathway and makes provision for industrial-scale synthesis of this valuable pharmaceutical compound.</p>

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Exploring cordycepin synthesis: comparative genomics analysis of ascomycetes fungi reveals insights into potential cordycepin production

  • 期刊论文

  • 发表日期：

    2026年3月9日

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  • Sreelakshmi Mundekkottil Venugopalan,
  • Anu Kalia,
  • Nitika Sandhu,
  • Aman Kumar,
  • Shivani Sharma

摘要

Cordyceps militaris, an entomopathogenic fungus is known for its numerous bioactive metabolites, particularly cordycepin. The biosynthetic pathway involved in cordycepin production is still elusive. There is a need to identify the existence of cordycepin biosynthesis genes in other ascomycetes fungal genera. This study explores this possibility through the evaluation of the occurrence of orthologs of cns1-3 genes which are essential for cordycepin production in Cordyceps militaris. The cns1-3 genomic and protein sequences of Cordyceps militaris were utilized to perform pBLAST and motif analysis, to identify ortholog genes in other ascomycetes fungi. These proteins were subjected to physiochemical characterization and their molecular functions were predicted. The multiple sequence alignment studies revealed variations as well as similarities in conserved domains of Cordyceps militaris and its orthologs. The protein motif studies further helped in prediction of the structural similarities for the secondary and tertiary structures. The results underline the cryptic nature of these secondary metabolite genes by demonstrating the existence of putative cordycepin synthesis proteins in other fungi. In vivo research studies, such as heterologous gene expression analyses can be beneficial to complete comprehension of the potential for cordycepin production in these fungi. These orthologs could be the putative candidates for verifying the new methods of gene elicitation for improved synthesis of bioactive metabolites. This study provides a valuable insight for diversification of cordycepin bio-synthesis pathway and makes provision for industrial-scale synthesis of this valuable pharmaceutical compound.