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<p><i>Acanthamoeba</i> is a genus of free-living amoebae found in both natural and artificial environments and it is of clinical importance due to its ability to cause serious diseases in humans and animals. The aim of this study was to monitor the occurrence of <i>Acanthamoeba</i> strains in the environment of Slovakia. A total of 16 environmental samples were studied using cytopathic, molecular-biological, and phylogenetic analyses. The cytopathic effect was observed in vitro on Vero cell cultures. Molecular analysis focused on the nuclear 18S ribosomal RNA (rRNA) gene. The genus was identified using a short fragment of ASA.S1 (&lt; 500&#xa0;bp), while near-full-length nuclear 18S rRNA gene sequences (&gt; 2200&#xa0;bp) were used for genotype determination and phylogenetic classification. To date, 23 genotypes (T1–T23) have been identified based on molecular analysis of the nuclear 18S rRNA gene. In this study, genotype T4 was the most prevalent (found in 9 samples), 6 samples were identified as genotype T15, and one isolate of genotype T18, which was detected for the first time in environmental samples from Slovakia.</p>

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Occurrence of Acanthamoeba spp. in the environment of Slovakia

  • 期刊论文

  • 发表日期：

    2026年1月13日

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  • Hana Némová,
  • Lucia Chomová,
  • Viera Nagyová

摘要

Acanthamoeba is a genus of free-living amoebae found in both natural and artificial environments and it is of clinical importance due to its ability to cause serious diseases in humans and animals. The aim of this study was to monitor the occurrence of Acanthamoeba strains in the environment of Slovakia. A total of 16 environmental samples were studied using cytopathic, molecular-biological, and phylogenetic analyses. The cytopathic effect was observed in vitro on Vero cell cultures. Molecular analysis focused on the nuclear 18S ribosomal RNA (rRNA) gene. The genus was identified using a short fragment of ASA.S1 (< 500 bp), while near-full-length nuclear 18S rRNA gene sequences (> 2200 bp) were used for genotype determination and phylogenetic classification. To date, 23 genotypes (T1–T23) have been identified based on molecular analysis of the nuclear 18S rRNA gene. In this study, genotype T4 was the most prevalent (found in 9 samples), 6 samples were identified as genotype T15, and one isolate of genotype T18, which was detected for the first time in environmental samples from Slovakia.