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<p>Plasmid-mediated quinolone resistance (PMQR) genes are widely present among <i>Enterobacterales,</i> leading to treatment failures, extended hospital stays, and increased mortality percentages. <i>qnrS</i> is one of important PMQR, which usually found in multi-resistance plasmids. However, limited information does exist about structure of <i>qnrS</i>1-harboring plasmid in China. The aim of the present study was to investigate the sequence of a novel conjugative <i>qnrS</i>1-harboring IncFIB plasmid (pFZ11). The plasmid was obtained from an <i>E. coli</i> sequence type 1312 strain previously identified in Fuzhou, China. pFZ11 was 79,147-bp in length and carried only one quinolone resistance gene, <i>qnrS</i>1. In addition, it contained two <i>β</i>-lactamase genes (<i>bla</i>TEM-1 and <i>bla</i>CTX-M-15), two streptomycin resistance genes (<i>strA</i> and <i>strB</i>), one trimethoprim resistance gene cassette (<i>dfrA</i>14), and a tetracycline resistance determinant (<i>tetA</i>). pFZ11 was transferred successfully into <i>E. coli</i> J53<sup>Ar</sup> at a conjugation frequency of 3.5 × 10<sup>–5</sup> ± 1.9 × 10<sup>–5</sup>. pFZ11 could be maintained at 100% for 15&#xa0;days without antibiotic stress. then pFZ11 gradually lost, but still remained above 94% until 30&#xa0;days. The plasmid pFZ11 was highly similar to plasmid pPGRT46 from Nigeria. <i>IS</i>2-<i>qnrS</i>1-<i>ISkra</i>4 module was found in pFZ11, which probably facilitates the mobilization of such quinolone resistance gene. Our study unveils one <i>qnrS</i>1-haboring IncFIB conjugative plasmid from <i>E. coli</i> clinical isolate in China, shedding light on its transferability, stability, and genetic structure.</p>

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Complete nucleotide sequence of qnrS1-haboring plasmid from E. coli clinical isolate in China

  • 期刊论文

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  • 发表日期：

    2026年1月15日

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  • Zhichang Zhao,
  • Yingbin Huang

摘要

Plasmid-mediated quinolone resistance (PMQR) genes are widely present among Enterobacterales, leading to treatment failures, extended hospital stays, and increased mortality percentages. qnrS is one of important PMQR, which usually found in multi-resistance plasmids. However, limited information does exist about structure of qnrS1-harboring plasmid in China. The aim of the present study was to investigate the sequence of a novel conjugative qnrS1-harboring IncFIB plasmid (pFZ11). The plasmid was obtained from an E. coli sequence type 1312 strain previously identified in Fuzhou, China. pFZ11 was 79,147-bp in length and carried only one quinolone resistance gene, qnrS1. In addition, it contained two β-lactamase genes (blaTEM-1 and blaCTX-M-15), two streptomycin resistance genes (strA and strB), one trimethoprim resistance gene cassette (dfrA14), and a tetracycline resistance determinant (tetA). pFZ11 was transferred successfully into E. coli J53Ar at a conjugation frequency of 3.5 × 10–5 ± 1.9 × 10–5. pFZ11 could be maintained at 100% for 15 days without antibiotic stress. then pFZ11 gradually lost, but still remained above 94% until 30 days. The plasmid pFZ11 was highly similar to plasmid pPGRT46 from Nigeria. IS2-qnrS1-ISkra4 module was found in pFZ11, which probably facilitates the mobilization of such quinolone resistance gene. Our study unveils one qnrS1-haboring IncFIB conjugative plasmid from E. coli clinical isolate in China, shedding light on its transferability, stability, and genetic structure.