Scholarlink

<p>Uropathogenic <i>Escherichia coli</i> (UPEC) is a major cause of urinary tract infections, causing treatment difficulties through extended spectrum ß-lactamase (ESBL) production and biofilm formation. This study assessed antimicrobial resistance, ESBL genes, and the effect of fosfomycin on biofilm formation in UPEC isolates obtained from patients at Amasya University Research and Training Hospital, Türkiye. A total of 93 <i>E. coli</i> isolates from urine samples were analyzed for antimicrobial susceptibility (disc diffusion), biofilm formation (microtiter assay), ESBL production [VITEK-2, combined disc test (CDT)], and ESBL genes (<i>bla</i><sub><i>TEM</i></sub>, <i>bla</i><sub><i>SHV</i></sub>, <i>bla</i><sub><i>CTX-M</i></sub><i>)</i> by polymerase chain reaction (PCR). The minimum biofilm inhibitory concentration of fosfomycin was tested on selected isolates using the microtiter plate method. The isolates exhibited resistance profiles of 100% to cefuroxime, 98.92% to ampicillin, 93.54% to ceftriaxone, 83.87% to ceftazidime, 65.59% to trimethoprim/sulfamethoxazole, 61.29% to ciprofloxacin, 56.98% to amoxicillin/clavulanic acid, 32.25% to gentamicin, and 24.73% to piperacillin/tazobactam, while susceptibility was observed to fosfomycin 100%, meropenem 100%, imipenem 100%, nitrofurantoin 98.92%, and amikacin 96.77%. All isolates were ESBL-positive by VITEK-2 and CDT. The prevalence of ESBL-encoding genes was determined as <i>bla</i><sub><i>TEM</i></sub> 26 (27.95%), <i>bla</i><sub><i>SHV</i></sub> 35 (37.63%), and <i>bla</i><sub><i>CTX-M</i></sub> 58 (62.36%). The <i>intI1</i> gene was detected in 57 isolates (61.29%). A correlation was identified between biofilm formation and the <i>bla</i><sub><i>TEM</i></sub> and <i>bla</i><sub><i>SHV</i></sub> genes (<i>p</i> &lt; 0.05). Biofilm production levels among the isolates were classified as strong (8.60%), moderate (19.35%), weak (29.03%), and non-biofilm producers (43.01%). Biofilm formation was inhibited by fosfomycin (1–8&#xa0;μg/mL) in eight strong biofilm-forming isolates. These results highlight the high prevalence of multidrug-resistant, ESBL-producing uropathogenic <i>E</i>. <i>coli</i> (UPEC) with strong biofilm-forming capacity and indicate that fosfomycin may serve as an effective anti-biofilm agent in the management of urinary tract infections.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Determination of extended spectrum beta-lactamase production in uropathogen Escherichia coli isolates and in vitro activity of fosfomycin on biofilm

  • 期刊论文

  • 发表日期：

    2026年1月8日

查看全文
  • Ceren Başkan,
  • Fikriye Milletli Sezgin,
  • Belgin Sırıken,
  • Adem Koçyiğit,
  • Ömer Ertürk

摘要

Uropathogenic Escherichia coli (UPEC) is a major cause of urinary tract infections, causing treatment difficulties through extended spectrum ß-lactamase (ESBL) production and biofilm formation. This study assessed antimicrobial resistance, ESBL genes, and the effect of fosfomycin on biofilm formation in UPEC isolates obtained from patients at Amasya University Research and Training Hospital, Türkiye. A total of 93 E. coli isolates from urine samples were analyzed for antimicrobial susceptibility (disc diffusion), biofilm formation (microtiter assay), ESBL production [VITEK-2, combined disc test (CDT)], and ESBL genes (blaTEM, blaSHV, blaCTX-M) by polymerase chain reaction (PCR). The minimum biofilm inhibitory concentration of fosfomycin was tested on selected isolates using the microtiter plate method. The isolates exhibited resistance profiles of 100% to cefuroxime, 98.92% to ampicillin, 93.54% to ceftriaxone, 83.87% to ceftazidime, 65.59% to trimethoprim/sulfamethoxazole, 61.29% to ciprofloxacin, 56.98% to amoxicillin/clavulanic acid, 32.25% to gentamicin, and 24.73% to piperacillin/tazobactam, while susceptibility was observed to fosfomycin 100%, meropenem 100%, imipenem 100%, nitrofurantoin 98.92%, and amikacin 96.77%. All isolates were ESBL-positive by VITEK-2 and CDT. The prevalence of ESBL-encoding genes was determined as blaTEM 26 (27.95%), blaSHV 35 (37.63%), and blaCTX-M 58 (62.36%). The intI1 gene was detected in 57 isolates (61.29%). A correlation was identified between biofilm formation and the blaTEM and blaSHV genes (p < 0.05). Biofilm production levels among the isolates were classified as strong (8.60%), moderate (19.35%), weak (29.03%), and non-biofilm producers (43.01%). Biofilm formation was inhibited by fosfomycin (1–8 μg/mL) in eight strong biofilm-forming isolates. These results highlight the high prevalence of multidrug-resistant, ESBL-producing uropathogenic E. coli (UPEC) with strong biofilm-forming capacity and indicate that fosfomycin may serve as an effective anti-biofilm agent in the management of urinary tract infections.