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<p>High-altitude aquatic ecosystems, particularly those associated with sacred and relatively pristine environments represent unique reservoirs of microbial diversity with potential agricultural significance. This study explores the diversity of phosphorus (P) and potassium (K) solubilizing bacteria (PSB and KSB) isolated from water samples in the lake of Highest Krishna Temple located in Yulla Kanda, Kinnaur district, Himachal Pradesh, India. Using selective enrichment techniques and specific culture media, a total of 41 morphologically distinct bacterial isolates were obtained. Screening on Pikovskaya’s and Aleksandrov agar media revealed 12 isolates capable of solubilizing inorganic phosphate and 15 isolates capable of solubilizing K- bearing minerals, as evidenced by the formation of clear halo zones. Quantitative assays in broth culture confirmed significant solubilization activity, with phosphate and potassium solubilization ranging from 28.06 ± 0.02 (mg L<sup>−1</sup>) to 287 ± 0.03 (mg L<sup>−1</sup>) and 36.19 ± 0.03 (mg L<sup>−1</sup>) to 367 ± 0.02 (mg L<sup>−1</sup>) respectively. Molecular identification through 16S rRNA gene sequencing revealed the presence of bacterial genera including <i>Bacillus, Pseudomonas, Azospirillum</i><i>, </i><i>Aeromonas, Enterobacter, Alcaligenes</i><i>, </i><i>Elizabethkingia</i><i>, </i><i>Glutamicibacter</i><i>, </i><i>Nocardia</i><i>, </i><i>Pectobacterium</i><i>, </i><i>Curtobacterium</i><i>, </i><i>Salinispora, Micrococcus</i> and <i>Chryseobacterium,</i> many of which are recognized for their plant growth promoting (PGP) capabilities. The microbial diversity observed in the cold-arid, oligotrophic water environment of Yulla Kanda suggests strong adaptive features and metabolic potential. This study is the first report on novel efficient PSB and KSB strains from high-altitude aquatic ecosystem and this sacred and high-altitude aquatic niche offers promising candidates for the development of biofertilizers suited to temperate and mountainous agro-ecosystems. This study also underscores the ecological and biotechnological value of underexplored high-altitude water bodies in India’s trans-Himalayan region.</p>

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First report of novel culturable psychrotolerant minerals solubilizing bacteria from high-altitude Yulla Kanda lake of Kinnaur Himalaya, India

  • 期刊论文

  • 发表日期：

    2026年1月13日

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  • Rajeshwari Negi,
  • Neelam Yadav,
  • Ajar Nath Yadav

摘要

High-altitude aquatic ecosystems, particularly those associated with sacred and relatively pristine environments represent unique reservoirs of microbial diversity with potential agricultural significance. This study explores the diversity of phosphorus (P) and potassium (K) solubilizing bacteria (PSB and KSB) isolated from water samples in the lake of Highest Krishna Temple located in Yulla Kanda, Kinnaur district, Himachal Pradesh, India. Using selective enrichment techniques and specific culture media, a total of 41 morphologically distinct bacterial isolates were obtained. Screening on Pikovskaya’s and Aleksandrov agar media revealed 12 isolates capable of solubilizing inorganic phosphate and 15 isolates capable of solubilizing K- bearing minerals, as evidenced by the formation of clear halo zones. Quantitative assays in broth culture confirmed significant solubilization activity, with phosphate and potassium solubilization ranging from 28.06 ± 0.02 (mg L−1) to 287 ± 0.03 (mg L−1) and 36.19 ± 0.03 (mg L−1) to 367 ± 0.02 (mg L−1) respectively. Molecular identification through 16S rRNA gene sequencing revealed the presence of bacterial genera including Bacillus, Pseudomonas, Azospirillum, Aeromonas, Enterobacter, Alcaligenes, Elizabethkingia, Glutamicibacter, Nocardia, Pectobacterium, Curtobacterium, Salinispora, Micrococcus and Chryseobacterium, many of which are recognized for their plant growth promoting (PGP) capabilities. The microbial diversity observed in the cold-arid, oligotrophic water environment of Yulla Kanda suggests strong adaptive features and metabolic potential. This study is the first report on novel efficient PSB and KSB strains from high-altitude aquatic ecosystem and this sacred and high-altitude aquatic niche offers promising candidates for the development of biofertilizers suited to temperate and mountainous agro-ecosystems. This study also underscores the ecological and biotechnological value of underexplored high-altitude water bodies in India’s trans-Himalayan region.