Scholarlink

<p><i>Fusarium proliferatum</i>, a fungal pathogen that causes wilt and dieback in date palms (<i>Phoenix dactylifera</i> L.), was isolated and molecularly characterized for the first time in southwestern Algeria. In parallel, the antifungal potential of halotolerant bacterial strains newly isolated from the saline soil of the Naama Sabkha was investigated as a sustainable approach to control this pathogen. Of the 110 bacterial isolates, 63 exhibited antifungal activity in agar disk assays. Eight strains (KLI 32, KLI 36, KLI 52, KLI 43, KLI 11, KLI 62, KLI 60, and KLI 59) significantly inhibited <i>F. proliferatum</i> growth in the dual-culture assay, with inhibition rates ranging from 45.23% to 56.92%. The most effective strain, KLI 32, restricted mycelial growth to 22&#xa0;mm, which corresponds to 56.92% inhibition. Microscopic examination of the interaction zone revealed marked hyphal deformities, suggesting direct antagonistic interaction. Cell-free supernatants derived from these strains inhibited fungal growth by 49.35–88.88% in the microdilution assay, with KLI 32 exhibiting the highest efficacy (88.88% inhibition rate). The inhibitory activity was dose-dependent and decreased progressively with dilution. Phenotypic, biochemical, and 16S rDNA sequence analyses identified all isolates selected for their significant antifungal potential against <i>F. proliferatum</i> as members of the genus <i>Bacillus</i>, capable of tolerating 0–25% (w/v) NaCl, with optimal growth observed at 10% NaCl. These results underscore the strong biocontrol potential of halotolerant <i>Bacillus</i> strains from Naama Sabkha against targeted phytopathogenic fungi, offering a promising and environmentally sustainable alternative to chemical fungicides.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Halotolerant Bacillus strains from the Sabkha of Naama, Algeria, as potential biocontrol agents against Fusarium proliferatum, the causal agent of date palm wilt and dieback

  • 期刊论文

  • 发表日期：

    2026年1月13日

查看全文
  • Leyla Miloudi-Agha,
  • Mohammed Kebdani,
  • Rida Mohammed Mediouni,
  • Sidi Mohammed Lahbib Seddiki,
  • Moustafa Yassine Mahdad,
  • Ibrahim Elkhalil Benzohra,
  • Abdeltif Amrane,
  • Lotfi Mouni,
  • Abdelwahab Rai

摘要

Fusarium proliferatum, a fungal pathogen that causes wilt and dieback in date palms (Phoenix dactylifera L.), was isolated and molecularly characterized for the first time in southwestern Algeria. In parallel, the antifungal potential of halotolerant bacterial strains newly isolated from the saline soil of the Naama Sabkha was investigated as a sustainable approach to control this pathogen. Of the 110 bacterial isolates, 63 exhibited antifungal activity in agar disk assays. Eight strains (KLI 32, KLI 36, KLI 52, KLI 43, KLI 11, KLI 62, KLI 60, and KLI 59) significantly inhibited F. proliferatum growth in the dual-culture assay, with inhibition rates ranging from 45.23% to 56.92%. The most effective strain, KLI 32, restricted mycelial growth to 22 mm, which corresponds to 56.92% inhibition. Microscopic examination of the interaction zone revealed marked hyphal deformities, suggesting direct antagonistic interaction. Cell-free supernatants derived from these strains inhibited fungal growth by 49.35–88.88% in the microdilution assay, with KLI 32 exhibiting the highest efficacy (88.88% inhibition rate). The inhibitory activity was dose-dependent and decreased progressively with dilution. Phenotypic, biochemical, and 16S rDNA sequence analyses identified all isolates selected for their significant antifungal potential against F. proliferatum as members of the genus Bacillus, capable of tolerating 0–25% (w/v) NaCl, with optimal growth observed at 10% NaCl. These results underscore the strong biocontrol potential of halotolerant Bacillus strains from Naama Sabkha against targeted phytopathogenic fungi, offering a promising and environmentally sustainable alternative to chemical fungicides.