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<p>This study reports the isolation and identification of a cellulase-producing&#xa0;Streptomyces strain from soil, characterized through&#xa0;morphological, biochemical, and molecular (16S-rRNA gene analysis)&#xa0;methods as&#xa0;<i>Streptomyces alfalfae</i>&#xa0;GHS22. The strain exhibited significant&#xa0;cellulolytic activity, with preliminary assays revealing optimal activity (29.32 U/mL) under specific conditions (pH 7, 25 °C, 1% CMC, 4-day incubation). Statistical approaches&#xa0;(Plackett–Burman design) identified&#xa0;CMC concentration, temperature, and incubation time&#xa0;as key factors influencing enzyme activity (<i>p</i> &lt; 0.05, R2 = 0.998). Further optimization using response surface methodology (RSM)&#xa0;yielded a cellulase activity of 52.15 U/mL under predicted optimal conditions (27.5 °C, 0.75% CMC, 2-day incubation). These results highlight the&#xa0;biotechnological potential&#xa0;of&#xa0;<i>S. alfalfae</i> GHS22 for industrial applications, particularly in&#xa0;biofuel and enzyme production, while contributing to the understanding of&#xa0;microbial physiology and environmental adaptation.</p>

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Polyphasic characterization and statistical optimization of cellulase production by Streptomyces alfalfae GHS22, a novel soil-derived Actinobacterium

  • 期刊论文

  • 发表日期：

    2025年10月13日

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  • Samia Gharbi,
  • Noureddine Karkachi,
  • Kamal Bendedouche,
  • Mehdi Adjdir

摘要

This study reports the isolation and identification of a cellulase-producing Streptomyces strain from soil, characterized through morphological, biochemical, and molecular (16S-rRNA gene analysis) methods as Streptomyces alfalfae GHS22. The strain exhibited significant cellulolytic activity, with preliminary assays revealing optimal activity (29.32 U/mL) under specific conditions (pH 7, 25 °C, 1% CMC, 4-day incubation). Statistical approaches (Plackett–Burman design) identified CMC concentration, temperature, and incubation time as key factors influencing enzyme activity (p < 0.05, R2 = 0.998). Further optimization using response surface methodology (RSM) yielded a cellulase activity of 52.15 U/mL under predicted optimal conditions (27.5 °C, 0.75% CMC, 2-day incubation). These results highlight the biotechnological potential of S. alfalfae GHS22 for industrial applications, particularly in biofuel and enzyme production, while contributing to the understanding of microbial physiology and environmental adaptation.