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<p>Carotenoid biosynthesis in <i>Dunaliella</i> species is regulated by a complex network of molecular mechanisms, which remain largely unexplored. MicroRNAs (miRNAs) function as post-transcriptional regulators, acting as molecular switches that modulate gene expression. However, their role in the regulation of carotenogenesis pathway genes (<i>Crt</i> genes) in <i>D. salina</i> has not been investigated. This study aimed to identify and characterize miRNAs involved in the regulation of <i>Crt</i> genes in <i>D. salina</i> using publicly available Sequence Read Archive (SRA) data and a homology-based mapping approach. A total of 30 miRNAs were mapped from the <i>D. salina</i> SRA dataset, and their potential targets among <i>Crt</i> genes were predicted using psRNATarget. The miRNA-target interactions were validated through RNA hybridization analysis using RNAHybrid. Five miRNAs—dsa-miR1163.2, dsa-miR917, dsa-miR1145.1, dsa-miR166a-3p, and dsa-miR414—were identified in <i>D. salina</i> as putative regulators of <i>Crt</i> genes and were further analyzed for differential expression under salinity stress using reverse transcription semi-quantitative and quantitative PCR (qPCR) was performed to confirm their expression profiles. This study represents the first report of miRNAs from a <i>D. salina</i> strain isolated from Sambhar Lake, India, providing novel insights into miRNA-mediated regulation of <i>Crt</i> genes. These findings pave the way for future research on engineering <i>D. salina</i> strains with enhanced carotenoid production for commercial applications.</p>

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Identification of microRNAs in Dunaliella salina and their potential role in carotenogenesis under salinity stress

  • 期刊论文

  • 发表日期：

    2025年7月28日

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  • Yogita Sharma,
  • Rekha Yadav,
  • Hemakshi Sharma,
  • Chandra Pal Singh

摘要

Carotenoid biosynthesis in Dunaliella species is regulated by a complex network of molecular mechanisms, which remain largely unexplored. MicroRNAs (miRNAs) function as post-transcriptional regulators, acting as molecular switches that modulate gene expression. However, their role in the regulation of carotenogenesis pathway genes (Crt genes) in D. salina has not been investigated. This study aimed to identify and characterize miRNAs involved in the regulation of Crt genes in D. salina using publicly available Sequence Read Archive (SRA) data and a homology-based mapping approach. A total of 30 miRNAs were mapped from the D. salina SRA dataset, and their potential targets among Crt genes were predicted using psRNATarget. The miRNA-target interactions were validated through RNA hybridization analysis using RNAHybrid. Five miRNAs—dsa-miR1163.2, dsa-miR917, dsa-miR1145.1, dsa-miR166a-3p, and dsa-miR414—were identified in D. salina as putative regulators of Crt genes and were further analyzed for differential expression under salinity stress using reverse transcription semi-quantitative and quantitative PCR (qPCR) was performed to confirm their expression profiles. This study represents the first report of miRNAs from a D. salina strain isolated from Sambhar Lake, India, providing novel insights into miRNA-mediated regulation of Crt genes. These findings pave the way for future research on engineering D. salina strains with enhanced carotenoid production for commercial applications.