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<p>The screening of thirty two bacterial isolates collected from dye contaminated soil for laccase production revealed the high laccase production potential of the isolate K14. The laccase-producing bacterial strains were tested with guaiacol as a substrate. The bacterial isolate was identified as <i>Lysinibacillus boronitolerans</i>. To optimize a low-cost medium for laccase synthesis, the following parameters were used: temperature (37&#xa0;°C), pH (7), inoculum (1%), incubation period (5&#xa0;days). The media's efficiency for laccase production was statistically evaluated. An artificial neural network predicted optimal laccase production in the response surface model (RSM), with a net regression value of 0.98. The optimal media conditions for laccase production were as follows: carbon source (glucose 2%), nitrogen source (peptone 2%), inducer (copper sulfate 0.4&#xa0;mM), pH (8), temperature (35&#xa0;°C), inoculum size (1.5%) and duration (2&#xa0;days). The minimal laccase production in the response surface model was 7.34 U/mL and the maximal laccase production was 12.23 U/mL on the 2nd day.</p>

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Optimization of laccase from Lysinibacillus boronitolerans using response surface methodology

  • 期刊论文

  • 发表日期：

    2025年7月15日

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  • Kamaljit Panchal,
  • Laxmi Bhatti,
  • Meenakshi Suhag,
  • Sunita Khatak,
  • Rajesh Kumar

摘要

The screening of thirty two bacterial isolates collected from dye contaminated soil for laccase production revealed the high laccase production potential of the isolate K14. The laccase-producing bacterial strains were tested with guaiacol as a substrate. The bacterial isolate was identified as Lysinibacillus boronitolerans. To optimize a low-cost medium for laccase synthesis, the following parameters were used: temperature (37 °C), pH (7), inoculum (1%), incubation period (5 days). The media's efficiency for laccase production was statistically evaluated. An artificial neural network predicted optimal laccase production in the response surface model (RSM), with a net regression value of 0.98. The optimal media conditions for laccase production were as follows: carbon source (glucose 2%), nitrogen source (peptone 2%), inducer (copper sulfate 0.4 mM), pH (8), temperature (35 °C), inoculum size (1.5%) and duration (2 days). The minimal laccase production in the response surface model was 7.34 U/mL and the maximal laccase production was 12.23 U/mL on the 2nd day.