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<p>Streptomyces species are abundant in the soil and serve as the main source of bioactive compounds. The aim of this study isolation and identification of native <i>Streptomyces spp.</i> in north of Iran and purification these compounds. Fifteen soil samples were collected from different area. These samples after serial dilution were pour in nutrition media for arise the bacterial colonies. After staining and colony purification, the <i>Streptomyces</i> were identified using biochemical method. Then, the identified <i>Streptomyces</i> were verified using molecular method with 16SrRNA sequencing. Phylogenetic analysis was conducted to investigate their kinship relationship. Their antibacterial effects were assessed by disk diffusion against clinical and standard strains of <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i>. Due to evaluation, one strain was selected with the most antibacterial effect to identify of bioactive compounds by GC–MS and LC–MS methods. The extracellular bioactive compounds were extracted by methanol and ethyl acetate solvents. The 16SrRNA gene sequence analysis showed the similarity of the isolated strain to Streptomyces microflavus with 93.92% confidence (EA6 strain). Antibacterial activity evaluations showed that ethyl acetate extract has more effective than methanolic extract. The minimum inhibitory concentration (MIC) of ethyl acetate on clinical and standard <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i> were from 1.25 to 5&#xa0;mg/mL. According to GC–MS analysis, acetic acid, 2-methylpropyl ester (17.65%) was identified as ethyl acetate extract main compound. Pursuant to LC–MS analysis of methanolic extract, tetracycline, dihydroxybenzamide, phenol, 2, 2'-methylenebis [6-(1,1-dimethyl ethyl)-4-methyl], and penipacid E were identified. The present research was showed that the strain EA6 has the great antibacterial activity potential for selected pathogens and it can be used as biomedical drug for combating multidrug-resistance bacteria<i>.</i></p>

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Evaluation of the antimicrobial activity of Streptomyces microflavus strain EA6 isolated from agricultural soils in northern Iran and identification of its bioactive compounds by GC–MS and LC–MS methods

  • 期刊论文

  • 发表日期：

    2025年7月7日

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  • Elham Amiri,
  • Mirsasan Mirpour,
  • Khosro Issazadeh,
  • Behnam Rasti

摘要

Streptomyces species are abundant in the soil and serve as the main source of bioactive compounds. The aim of this study isolation and identification of native Streptomyces spp. in north of Iran and purification these compounds. Fifteen soil samples were collected from different area. These samples after serial dilution were pour in nutrition media for arise the bacterial colonies. After staining and colony purification, the Streptomyces were identified using biochemical method. Then, the identified Streptomyces were verified using molecular method with 16SrRNA sequencing. Phylogenetic analysis was conducted to investigate their kinship relationship. Their antibacterial effects were assessed by disk diffusion against clinical and standard strains of Staphylococcus aureus and Pseudomonas aeruginosa. Due to evaluation, one strain was selected with the most antibacterial effect to identify of bioactive compounds by GC–MS and LC–MS methods. The extracellular bioactive compounds were extracted by methanol and ethyl acetate solvents. The 16SrRNA gene sequence analysis showed the similarity of the isolated strain to Streptomyces microflavus with 93.92% confidence (EA6 strain). Antibacterial activity evaluations showed that ethyl acetate extract has more effective than methanolic extract. The minimum inhibitory concentration (MIC) of ethyl acetate on clinical and standard Staphylococcus aureus and Pseudomonas aeruginosa were from 1.25 to 5 mg/mL. According to GC–MS analysis, acetic acid, 2-methylpropyl ester (17.65%) was identified as ethyl acetate extract main compound. Pursuant to LC–MS analysis of methanolic extract, tetracycline, dihydroxybenzamide, phenol, 2, 2'-methylenebis [6-(1,1-dimethyl ethyl)-4-methyl], and penipacid E were identified. The present research was showed that the strain EA6 has the great antibacterial activity potential for selected pathogens and it can be used as biomedical drug for combating multidrug-resistance bacteria.