<p>In this research, a fungal strain extracted from soil served as the basis for carboxymethyl cellulase (CMCase) production via submerged fermentation. The fungus was identified through 16S rRNA sequencing as <i>P. chrysogenum.</i> Employing the Central Composite Rotational Design (CCRD), the optimization of pH, incubation period, and inoculum quantity was carried out to enhance enzyme production. The optimal enzyme activity of 3.9 ± 0.1&#xa0;IU/mL was obtained at optimal variables of pH 5.5, incubation time 120&#xa0;h, inoculum size 6.5%, and temperature 40°C. Lactose and ammonium sulfate were the most effective carbon and nitrogen sources respectively at a specific C/N ratio (3.5) for. Zn²⁺ and ascorbic act as enhancers for cellulase production. Specific growth rate (μ) 3.44&#xa0;h⁻¹ and Monod's constant (K_s) 0.099 were determined using Monod′ kinetic equation. Values of product formation kinetics α, and β were 0.9682 and 1.7809 respectively with an R² value of 0.9421. The efficiency of crude CMCase was explored for hydrolysis of acid-treated wheat husk and saccharification of 42.02% was achieved under optimal conditions. The study unveils CMCase production by <i>P. chrysogenum</i> through submerged fermentation, shedding light on its kinetics, growth efficiency, and promising application in agrowaste bioconversion.&#xa0;</p> Graphical Abstract <p>Graphical abstract for carboxymethylcellulase (CMCase) production from newly isolated Penicillium chrysogenum and its application in wheat husk saccharification</p> <p></p>