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<p>Worldwide, Bt hybrids (genetically modified plants expressing <i>Bacillus thuringiensis</i> toxins) are widely used in agricultural crops, particularly cotton, as a modern technique to control insect pests. In India, the widespread cultivation of Bt cotton and excessive use of insecticides aimed at controlling the pink bollworm <i>Pectinophora gossypiella</i> (Saunders) (Lepidoptera: Gelechiidae) have led to the development of resistance. In insects, target mutations and detoxifying enzymes play key roles in the development of resistance to <i>Bt</i> and insecticides. Gut symbionts may play a key role in the resistance of insect pests to both insecticides and Bt cotton. The gut microbiota of pink bollworm populations was investigated via <i>16S rRNA</i> gene sequencing of total DNA from the midgut. Various strains of endosymbionts have been identified, with <i>Burkholderia</i> strains being notably predominant across multiple locations. Confirmation was conducted via the use of <i>Burkholderia</i>-specific primers to amplify gyrB gene fragments. Additionally, core bacterial communities such as <i>Pluralibacter gergoviae</i>, <i>Enterococcus gallinarum, Enterobacter hormaechei</i> and <i>Gossypium arboreum</i> were identified in larvae from certain locations. This study is the first to document <i>Burkholderia</i> spp. as endosymbionts in <i>P. gossypiella</i> from India. The symbiotic relationship with <i>Burkholderia</i> sp. may play a crucial role in the development of resistance to insecticides and Bt cotton, as well as in host physiology and nutrient assimilation. Further research on the role of this endosymbiont and its interactions could aid in the development of novel strategies to manage this emerging cotton pest.</p>

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Investigating gut bacteria in field populations of pink bollworm, Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae), in India

  • 期刊论文

  • 发表日期：

    2025年3月10日

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  • Vakudavath Chinna Babu Naik,
  • Pratik Prabodh Pusadkar,
  • Narayanappa Chandrashekar,
  • Arns Subbanna,
  • Jasti Sri Vishnu Murthy,
  • Lingutla Rajesh Chowdary,
  • Nandini Gokte Narkhedkar,
  • Yenumula Gerad Prasad

摘要

Worldwide, Bt hybrids (genetically modified plants expressing Bacillus thuringiensis toxins) are widely used in agricultural crops, particularly cotton, as a modern technique to control insect pests. In India, the widespread cultivation of Bt cotton and excessive use of insecticides aimed at controlling the pink bollworm Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae) have led to the development of resistance. In insects, target mutations and detoxifying enzymes play key roles in the development of resistance to Bt and insecticides. Gut symbionts may play a key role in the resistance of insect pests to both insecticides and Bt cotton. The gut microbiota of pink bollworm populations was investigated via 16S rRNA gene sequencing of total DNA from the midgut. Various strains of endosymbionts have been identified, with Burkholderia strains being notably predominant across multiple locations. Confirmation was conducted via the use of Burkholderia-specific primers to amplify gyrB gene fragments. Additionally, core bacterial communities such as Pluralibacter gergoviae, Enterococcus gallinarum, Enterobacter hormaechei and Gossypium arboreum were identified in larvae from certain locations. This study is the first to document Burkholderia spp. as endosymbionts in P. gossypiella from India. The symbiotic relationship with Burkholderia sp. may play a crucial role in the development of resistance to insecticides and Bt cotton, as well as in host physiology and nutrient assimilation. Further research on the role of this endosymbiont and its interactions could aid in the development of novel strategies to manage this emerging cotton pest.