Scholarlink

<p>Basil (<i>Ocimum basilicum</i>) is an edible herb threatened by various pests and diseases. Nematodes are known as one of the constraints on crop production. In this study, the response of 14 ecotypes of basil collected from all over Iran was assessed to root-knot nematode (<i>Meloidogyne incognita</i>) infection. By quantifying the number of galls and nematodes, it was demonstrated that ecotypes Birjand Sabz 11, Birjand Sabz 32 and Mobarakeh were the most susceptible ones to nematode infection while ecotypes Varamin 32, Isfahan 412 and Isfahan 221, were the less susceptible ecotypes in a completely randomized design experimental setup, and according to Tukey’s test at <i>P</i> ≤ 0.05. The less susceptible and most susceptible ecotypes, Varamin 32 and Birjand Sabz 11, respectively, were selected for further investigations. Both less susceptible and susceptible ecotypes failed to stimulate the activity of catalase and peroxidase at 24&#xa0;h post-inoculation (hpi) in roots. However, less susceptible plants could activate a defense response at 48 hpi and remained active at 72 hpi, whereas the susceptible ecotype failed to mount the defense response. Next to observing the antioxidative changes upon nematode infection, to further survey the plant defense responses in roots, the gene expression analyses using RT-qPCR revealed that at 48 hpi, <i>pathogenesis-related protein 5</i> (<i>PR-5</i>), <i>Thaumatin-like protein</i> (<i>TLP</i>), <i>Ascorbate </i><i> oxidase</i> (<i>AO</i>) and <i>Phenylalanine ammonia-lyase</i> (<i>PAL</i>), substantially increased in the less susceptible ecotype, whereas the susceptible ecotype showed no comparable response. Results demonstrated a significant variation in susceptibility to <i>Meloidogyne incognita</i> among 14 Iranian basil ecotypes, and that differential responses were not linked to geographic origin but rather to distinct antioxidative and molecular defense mechanisms. This study provides new insight into the mechanisms involved in basil defense against the root-knot nematode infection.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Induced antioxidative and pathogenesis-related defenses confer less susceptibility to root-knot nematode infection in basil ecotypes

  • 期刊论文

  • 发表日期：

    2026年5月6日

查看全文
  • Zahra Khosravian,
  • Mehdi Saadati,
  • Majid Pedram,
  • Mohammad Reza Atighi,
  • Masoud Shams-Bakhsh

摘要

Basil (Ocimum basilicum) is an edible herb threatened by various pests and diseases. Nematodes are known as one of the constraints on crop production. In this study, the response of 14 ecotypes of basil collected from all over Iran was assessed to root-knot nematode (Meloidogyne incognita) infection. By quantifying the number of galls and nematodes, it was demonstrated that ecotypes Birjand Sabz 11, Birjand Sabz 32 and Mobarakeh were the most susceptible ones to nematode infection while ecotypes Varamin 32, Isfahan 412 and Isfahan 221, were the less susceptible ecotypes in a completely randomized design experimental setup, and according to Tukey’s test at P ≤ 0.05. The less susceptible and most susceptible ecotypes, Varamin 32 and Birjand Sabz 11, respectively, were selected for further investigations. Both less susceptible and susceptible ecotypes failed to stimulate the activity of catalase and peroxidase at 24 h post-inoculation (hpi) in roots. However, less susceptible plants could activate a defense response at 48 hpi and remained active at 72 hpi, whereas the susceptible ecotype failed to mount the defense response. Next to observing the antioxidative changes upon nematode infection, to further survey the plant defense responses in roots, the gene expression analyses using RT-qPCR revealed that at 48 hpi, pathogenesis-related protein 5 (PR-5), Thaumatin-like protein (TLP), Ascorbate oxidase (AO) and Phenylalanine ammonia-lyase (PAL), substantially increased in the less susceptible ecotype, whereas the susceptible ecotype showed no comparable response. Results demonstrated a significant variation in susceptibility to Meloidogyne incognita among 14 Iranian basil ecotypes, and that differential responses were not linked to geographic origin but rather to distinct antioxidative and molecular defense mechanisms. This study provides new insight into the mechanisms involved in basil defense against the root-knot nematode infection.