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<p>This study investigates the enzymatic characteristics and interactions of trypsin and α-amylase from <i>Rhyzopertha dominica</i> and respective inhibitors from infested wheat lines during storage. We hypothesize that chromosomal segmental substitution lines (CSSLs) of wheat with higher initial concentrations of α-amylase and trypsin inhibitors maintain greater resistance against&#xa0;<i>Rhyzopertha dominica</i>&#xa0;over the storage periods; a defense that can be further improved by the application of exogenous inhibitors. A significant decline in trypsin and α-amylase inhibitor content was observed across wheat lines during prolonged storage, with PN 399 showing the least reduction, indicating their resilience behaviour. Trypsin activity peaked at a pH of 10.4 and showed maximum substrate affinity at 2 mM BApNA. Effective inhibitors of trypsin activity included Aprotinin and Leupeptin, reducing activity by up to 50%. Activators such as MgCl₂, CuSO₄, and CaCl₂ enhanced trypsin activity, while EDTA exhibited minimal effects. The α-amylase from <i>Rhyzopertha dominica</i> displayed an optimal pH of 7.0 and a optimum temperature of 40 °C, with maximum activity observed at 2% starch. Partial purification revealed peak enzyme activity in protein fractions from 30 to 60% ammonium sulfate saturation. Effective inhibitors like citric and oxalic acids reduced α-amylase activity significantly, while metal ions such as CaCl₂, NaCl, and MgCl₂ enhanced activity. The findings provide insights into enzyme kinetics and inhibitors, contributing to pest management strategies and the biochemical understanding of <i>Rhyzopertha dominica</i> in stored wheat.</p>

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The role of wheat-derived inhibitors in suppressing Rhyzopertha dominica infestation

  • 期刊论文

  • 发表日期：

    2026年3月4日

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  • Divjot Kaur,
  • Rachana D. Bhardwaj,
  • Pooja Rani,
  • Satvir Kaur Grewal,
  • Beant Singh,
  • Ritu Tandon,
  • Satinder Kaur

摘要

This study investigates the enzymatic characteristics and interactions of trypsin and α-amylase from Rhyzopertha dominica and respective inhibitors from infested wheat lines during storage. We hypothesize that chromosomal segmental substitution lines (CSSLs) of wheat with higher initial concentrations of α-amylase and trypsin inhibitors maintain greater resistance against Rhyzopertha dominica over the storage periods; a defense that can be further improved by the application of exogenous inhibitors. A significant decline in trypsin and α-amylase inhibitor content was observed across wheat lines during prolonged storage, with PN 399 showing the least reduction, indicating their resilience behaviour. Trypsin activity peaked at a pH of 10.4 and showed maximum substrate affinity at 2 mM BApNA. Effective inhibitors of trypsin activity included Aprotinin and Leupeptin, reducing activity by up to 50%. Activators such as MgCl₂, CuSO₄, and CaCl₂ enhanced trypsin activity, while EDTA exhibited minimal effects. The α-amylase from Rhyzopertha dominica displayed an optimal pH of 7.0 and a optimum temperature of 40 °C, with maximum activity observed at 2% starch. Partial purification revealed peak enzyme activity in protein fractions from 30 to 60% ammonium sulfate saturation. Effective inhibitors like citric and oxalic acids reduced α-amylase activity significantly, while metal ions such as CaCl₂, NaCl, and MgCl₂ enhanced activity. The findings provide insights into enzyme kinetics and inhibitors, contributing to pest management strategies and the biochemical understanding of Rhyzopertha dominica in stored wheat.