Promotion of growth and biochemical changes in the maize seedlings after pre-sowing treatment of seeds with oxygen plasma
摘要
Effect of pre sowing treatment of maize (Zea mays variety: GSF.2) seeds with Oxygen plasma has been investigated for the promotion of growth and the early biochemical changes. Oxygen plasma was produced in a RF plasma machine for exposing the seeds. A dose response curve in terms of energy levels and time of exposure to Oxygen plasma showed an optimum impact on growth at 30 W after 240 s of exposure. The activity of α-amylase was enhanced by Oxygen plasma during germination and early seedling growth. The expression of α-amylase gene was also enhanced by many folds. This was accompanied by a rise in the level of H₂O₂. It is concluded that H₂O₂ acts as a signaling molecule in maize seeds for enhancement of the activity of α-amylase by Oxygen plasma.