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<p><i>Mentha</i> Species are well known for their valuable essential oils. Limonene synthase is a key enzyme in the monoterpene biosynthesis pathway of mint. In this study, qRT-PCR analysis was conducted on various tissues and treatments of <i>Mentha canadensis</i> to reveal the expression levels and expression response patterns of limonene synthase (<i>McLS)</i> gene. The results demonstrated that <i>McLS</i> was highly expressed in young leaves and was induced by light, abscisic acid (ABA), methyl jasmonate (MeJA), NaCl, and mannitol treatments. The (-)-limonene synthase promoter (<i>proMcLS</i>) was isolated and its <i>cis</i> elements were analyzed. The upstream region contains several <i>cis</i>-acting elements, including core motifs such as the TATA box and CAAT box, light-responsive motifs, ABA- and MeJA-responsive motifs, and guard cell-specific <i>cis</i> elements. Transcriptional fusion of the <i>proMcLS</i> to the <i>gusA</i> reporter gene was conducted in <i>N</i>. <i>tabacum</i> via <i>Agrobacterium-</i>mediated transformation. Transgenic T0 lines displayed β-glucuronidase histochemical staining activity in short glandular trichomes and the stigma of flowers. No signal was detected in tall glandular trichomes or stomatal guard cells, however, T1 lines displayed β-glucuronidase activity in both short glandular trichomes and stomatal guard cells. Transcription factor families predicted to the <i>McLS</i> promoter were predicted using PlantPAN 3.0, and transcription factors co-expressed with <i>McLS</i> under various light treatments were identified. These findings describe a tissue-specific promoter that may be utilized for future metabolic engineering in plants.</p>

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The promoter sequence of (-)-limonene synthase in Mentha Canadensis and its strong activity in the glandular trichome and in the stomatal guard cells

  • 期刊论文

  • 发表日期：

    2026年1月16日

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  • Shumin Li,
  • Zhichao Xue,
  • Taolan Xiao,
  • Xiwu Qi,
  • Hailing Fang,
  • Li Li,
  • Yang Bai,
  • Dongmei Liu,
  • Qun Liu,
  • Zequn Chen,
  • Xu Yu,
  • Chengyuan Liang

摘要

Mentha Species are well known for their valuable essential oils. Limonene synthase is a key enzyme in the monoterpene biosynthesis pathway of mint. In this study, qRT-PCR analysis was conducted on various tissues and treatments of Mentha canadensis to reveal the expression levels and expression response patterns of limonene synthase (McLS) gene. The results demonstrated that McLS was highly expressed in young leaves and was induced by light, abscisic acid (ABA), methyl jasmonate (MeJA), NaCl, and mannitol treatments. The (-)-limonene synthase promoter (proMcLS) was isolated and its cis elements were analyzed. The upstream region contains several cis-acting elements, including core motifs such as the TATA box and CAAT box, light-responsive motifs, ABA- and MeJA-responsive motifs, and guard cell-specific cis elements. Transcriptional fusion of the proMcLS to the gusA reporter gene was conducted in N. tabacum via Agrobacterium-mediated transformation. Transgenic T0 lines displayed β-glucuronidase histochemical staining activity in short glandular trichomes and the stigma of flowers. No signal was detected in tall glandular trichomes or stomatal guard cells, however, T1 lines displayed β-glucuronidase activity in both short glandular trichomes and stomatal guard cells. Transcription factor families predicted to the McLS promoter were predicted using PlantPAN 3.0, and transcription factors co-expressed with McLS under various light treatments were identified. These findings describe a tissue-specific promoter that may be utilized for future metabolic engineering in plants.