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<p>CRISPR/Cas9 technology has revolutionized plant science as a precise and efficient genome-editing tool. By enabling targeted modifications in specific genes and genomic regions, it has accelerated functional genomics and facilitated the identification of genes associated with complex agronomic traits such as drought tolerance, pest resistance, and improved nutritional content. Compared with traditional approaches, CRISPR/Cas9 provides rapid functional validation through targeted mutagenesis, supporting both gene discovery and trait improvement. Its versatility extends beyond model systems to major crops and wild relatives, thereby expanding opportunities for plant breeding and conservation. Recent advances, including base and prime editing, have further broadened its scope, enabling high-resolution dissection of regulatory networks and genetic pathways. By integrating genome editing into plant research, CRISPR/Cas9 offers powerful avenues for developing climate-resilient and high-yielding crops, contributing to global food security and sustainable agriculture.</p>

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Mapping the plant genome: CRISPR/Cas9 as a toolkit for functional genomics

  • 期刊论文

  • 发表日期：

    2025年12月15日

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  • Samar G. Thabet,
  • Amr Elkelish,
  • Mona F. A. Dawood,
  • Fatmah Ahmed Safhi,
  • Abdulrahman M. Alhudhaibi,
  • Andreas Börner,
  • Ahmad M. Alqudah

摘要

CRISPR/Cas9 technology has revolutionized plant science as a precise and efficient genome-editing tool. By enabling targeted modifications in specific genes and genomic regions, it has accelerated functional genomics and facilitated the identification of genes associated with complex agronomic traits such as drought tolerance, pest resistance, and improved nutritional content. Compared with traditional approaches, CRISPR/Cas9 provides rapid functional validation through targeted mutagenesis, supporting both gene discovery and trait improvement. Its versatility extends beyond model systems to major crops and wild relatives, thereby expanding opportunities for plant breeding and conservation. Recent advances, including base and prime editing, have further broadened its scope, enabling high-resolution dissection of regulatory networks and genetic pathways. By integrating genome editing into plant research, CRISPR/Cas9 offers powerful avenues for developing climate-resilient and high-yielding crops, contributing to global food security and sustainable agriculture.