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<p>Ethylene responsive factors (ERFs) play pivotal roles in regulating plant response to environmental stresses. An increasing body of studies have been published on characterizing the function of ERFs in plants. However, there are very few reports regarding stress-related ERF proteins available in tomato. Here, the function of SlAP2e, a nucleus-localized transcription factor from tomato, in plant immune response against pathogens was characterized. Expression of <i>SlAP2e</i> could be induced by MeJA, ACC and SA. Transcriptional activation assays using yeast two-hybrid system demonstrated that SlAP2e functions as a transcriptional activator in yeast. Ectopic expression of <i>SlAP2e</i> in <i>Arabidopsis</i> confers increased resistance to necrotrophic fungus <i>Botrytis cinerea</i> but decreased resistance to hemibiotrophic bacteria <i>Pseudomonas syringae</i> pv. <i>tomato</i> DC3000. <i>SlAP2e</i> transgenic plants showed improved ROS-scavenging ability under H<sub>2</sub>O<sub>2</sub> stress. Real-time RT-PCR results indicated that pathogenesis-related (PR) genes (<i>AtPR1</i> and <i>AtPR5</i>) respond differentially to <i>Botrytis cinerea</i> and <i>Pseudomonas syringae</i> pv. <i>tomato</i> DC3000. All research data demonstrate that SlAP2e acts as an immune regulator that may endow the plants with differential responses to necrotrophic and biotrophic pathogens in <i>Arabidopsis</i>. SlAP2e can have potential beneficial effects in the genetic improvement of economically important crops.</p>

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Tomato SlAP2e differentially regulates plant basal immunity against Botrytis cinerea and Pseudomonas syringae pv. tomato DC3000

  • 期刊论文

  • 发表日期：

    2025年11月27日

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  • Lili Zhang,
  • Kaige Liu,
  • Zongwen Zhu,
  • Yuelu Zhao,
  • Yunpeng Song

摘要

Ethylene responsive factors (ERFs) play pivotal roles in regulating plant response to environmental stresses. An increasing body of studies have been published on characterizing the function of ERFs in plants. However, there are very few reports regarding stress-related ERF proteins available in tomato. Here, the function of SlAP2e, a nucleus-localized transcription factor from tomato, in plant immune response against pathogens was characterized. Expression of SlAP2e could be induced by MeJA, ACC and SA. Transcriptional activation assays using yeast two-hybrid system demonstrated that SlAP2e functions as a transcriptional activator in yeast. Ectopic expression of SlAP2e in Arabidopsis confers increased resistance to necrotrophic fungus Botrytis cinerea but decreased resistance to hemibiotrophic bacteria Pseudomonas syringae pv. tomato DC3000. SlAP2e transgenic plants showed improved ROS-scavenging ability under H2O2 stress. Real-time RT-PCR results indicated that pathogenesis-related (PR) genes (AtPR1 and AtPR5) respond differentially to Botrytis cinerea and Pseudomonas syringae pv. tomato DC3000. All research data demonstrate that SlAP2e acts as an immune regulator that may endow the plants with differential responses to necrotrophic and biotrophic pathogens in Arabidopsis. SlAP2e can have potential beneficial effects in the genetic improvement of economically important crops.