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<p>Iron (Fe) and sulphur (S) interactions have been widely studied, however there is little known about their interactive influence on other nutrients uptake, translocation and assimilation. The present study deciphered the role of Fe and S nutrition on carbon (C), nitrogen (N) and S uptake, assimilation and amino acid synthesis in bread and durum wheat through pot and nutrient culture experiments. The pot experiment was conducted with Fe deficient (Fe-, ~ 6.6&#xa0;kg Fe ha<sup>−1</sup>) and Fe sufficient (Fe + , ~ 26.4&#xa0;kg Fe ha<sup>−1</sup>) field soil with three levels of S i.e., 0 (S0), 30 (S1) and 60 (S2) kg S ha<sup>−1</sup> over two experimental years. Results showed that the Fe and S supply significantly increased shoot mass, photosynthesis, nutrient content, cysteine and methionine content and activities of key assimilating enzymes of C, N and S metabolism i.e. Rubisco, nitrate reductase (NR), serine acetyl transferase (SAT) and O-acetyl serine thiolyase (OASTL) respectively as compared to Fe-S0 treatment. Further, for the transcript expression of S (<i>SULTR1;1</i> and <i>SULTR2;1</i>) and N (<i>NRT2.1</i>) uptake transporters, plants were raised under Fe- (1&#xa0;µM) and Fe + (100&#xa0;µM) nutrient solutions with three S levels viz., 0 (S0), 1.25 (S1) and 2.5 (S2) mM, equivalent to the pot culture experiment. <i>SULTR1;1</i> expression was significantly induced under Fe + S0 condition whereas, viceversa results were observed in <i>SULTR2;1</i> expression. <i>NRT2.1</i> expression was induced under Fe- but with S supply. The study clearly reveals the interactive modulation of C-N-S-Fe uptake and metabolism by Fe and S supply in wheat.</p>

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Interactive modulation of carbon, nitrogen and sulphur assimilation under individual and combined deficiency of iron and sulphur determines growth, nutrient availability-uptake and synthesis of amino acids in bread and durum wheat

  • 期刊论文

  • 发表日期：

    2025年11月14日

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  • Vasundhara Sharma,
  • Pradeep Kumar Jain,
  • Shalini Gaur Rudra,
  • Yashbir Singh Shivay,
  • Anjali Anand,
  • Usha Kalidindi,
  • Bhupinder Singh

摘要

Iron (Fe) and sulphur (S) interactions have been widely studied, however there is little known about their interactive influence on other nutrients uptake, translocation and assimilation. The present study deciphered the role of Fe and S nutrition on carbon (C), nitrogen (N) and S uptake, assimilation and amino acid synthesis in bread and durum wheat through pot and nutrient culture experiments. The pot experiment was conducted with Fe deficient (Fe-, ~ 6.6 kg Fe ha−1) and Fe sufficient (Fe + , ~ 26.4 kg Fe ha−1) field soil with three levels of S i.e., 0 (S0), 30 (S1) and 60 (S2) kg S ha−1 over two experimental years. Results showed that the Fe and S supply significantly increased shoot mass, photosynthesis, nutrient content, cysteine and methionine content and activities of key assimilating enzymes of C, N and S metabolism i.e. Rubisco, nitrate reductase (NR), serine acetyl transferase (SAT) and O-acetyl serine thiolyase (OASTL) respectively as compared to Fe-S0 treatment. Further, for the transcript expression of S (SULTR1;1 and SULTR2;1) and N (NRT2.1) uptake transporters, plants were raised under Fe- (1 µM) and Fe + (100 µM) nutrient solutions with three S levels viz., 0 (S0), 1.25 (S1) and 2.5 (S2) mM, equivalent to the pot culture experiment. SULTR1;1 expression was significantly induced under Fe + S0 condition whereas, viceversa results were observed in SULTR2;1 expression. NRT2.1 expression was induced under Fe- but with S supply. The study clearly reveals the interactive modulation of C-N-S-Fe uptake and metabolism by Fe and S supply in wheat.