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<p>Salt stress significantly inhibits seed germination in tobacco, yet the molecular mechanisms underlying this process remain largely unexplored. In this study, we demonstrated that <i>NtOGG1</i>, encoding an 8-oxoguanine DNA glycosylase, functions as a positive regulator of salt tolerance during seed germination. Under 150&#xa0;mM NaCl stress, the overexpressing <i>NtOGG1</i> line (<i>NtOGG1</i>-OE) exhibited higher germination and seedling percentage compared to wildtype (WT), whereas CRISPR–Cas9 knockout mutant showed reductions in both parameters. RNA-Seq analysis revealed that differentially expressed genes (DEGs) in the <i>NtOGG1</i>-OE line, including ethylene-responsive transcription factors (ERFs), respiratory burst oxidase homologs (RBOHs), and catalase (CAT) genes, are implicated in the mitogen-activated protein kinase (MAPK) signaling pathway. Further qRT-PCR and physiological assays confirmed that the enhanced ethylene responses and reduced accumulation of reactive oxygen species (ROS) significantly promote seed germination in the <i>NtOGG1</i>-OE line under salt stress. These findings establish <i>NtOGG1</i> as a key regulator influencing seed germination under salt stress, providing a promising molecular target for breeding salt-tolerant tobacco cultivars.</p>

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NtOGG1 regulates tobacco seed germination involving ethylene and reactive oxygen species pathways under salt stress

  • 期刊论文

  • 发表日期：

    2025年9月13日

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  • Yongzhi Niu,
  • Wenlong Suo,
  • Guoping Wang,
  • Chengjing Wang,
  • Dandan Wang,
  • Zepeng Wu,
  • Zhoufei Wang,
  • Yunye Zheng

摘要

Salt stress significantly inhibits seed germination in tobacco, yet the molecular mechanisms underlying this process remain largely unexplored. In this study, we demonstrated that NtOGG1, encoding an 8-oxoguanine DNA glycosylase, functions as a positive regulator of salt tolerance during seed germination. Under 150 mM NaCl stress, the overexpressing NtOGG1 line (NtOGG1-OE) exhibited higher germination and seedling percentage compared to wildtype (WT), whereas CRISPR–Cas9 knockout mutant showed reductions in both parameters. RNA-Seq analysis revealed that differentially expressed genes (DEGs) in the NtOGG1-OE line, including ethylene-responsive transcription factors (ERFs), respiratory burst oxidase homologs (RBOHs), and catalase (CAT) genes, are implicated in the mitogen-activated protein kinase (MAPK) signaling pathway. Further qRT-PCR and physiological assays confirmed that the enhanced ethylene responses and reduced accumulation of reactive oxygen species (ROS) significantly promote seed germination in the NtOGG1-OE line under salt stress. These findings establish NtOGG1 as a key regulator influencing seed germination under salt stress, providing a promising molecular target for breeding salt-tolerant tobacco cultivars.