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Objective <p>To investigate the effect of mild moxibustion on transient receptor potential vanilloid type 1 (TRPV1) channel expression in primary dysmenorrhea (PD) rats and explore its mechanism in alleviating central pain sensitization.</p> Methods <p>Thirty-two female non-pregnant Wistar rats were randomized into a blank group, a model group, a mild moxibustion group, and a capsazepine group, with 8 rats in each group. Except for the blank group, the other three groups used estradiol benzoate, ice-water bath, and oxytocin to establish the rat PD model of cold-dampness stagnation pattern. The interventions began on day 1 of modeling, once a day, and lasted 10 d. The mild moxibustion group received mild moxibustion at Shenque (CV8) and Guanyuan (CV4), 20 min/time; in the capsazepine group, capsazepine was injected at a dose of 2 mg/(kg·bw). The abdominal pain threshold was measured 10–30 min after oxytocin injection on day 11; enzyme-linked immunosorbent assay was used to detect serum prostaglandin F<sub>2α</sub> (PGF<sub>2α</sub>) level; the expression of TRPV1, cluster of differentiation 11B (CD11B), and proto-oncogene c-Fos in the spinal dorsal horn and hypothalamus was detected by immunofluorescence and Western blotting.</p> Results <p>Compared to the blank group, the model group showed a decreased pain threshold (<i>P</i>&lt;0.05) and an increased serum PGF<sub>2α</sub> level with elevated TRPV1, CD11B, and c-Fos protein expression in the spinal dorsal horn and hypothalamus (<i>P</i>&lt;0.05). Compared to the model group, both the mild moxibustion group and capsazepine group showed significantly increased pain thresholds (<i>P</i>&lt;0.05), along with decreased serum PGF<sub>2α</sub> levels and reduced protein expression levels of TRPV1, CD11B, and c-Fos in the spinal dorsal horn and hypothalamus (<i>P</i>&lt;0.05). Rat pain threshold in the capsazepine group was higher than that in the mild moxibustion group (<i>P</i>&lt;0.05). Serum PGF<sub>2α</sub> level, the expression levels of CD11B and c-Fos proteins in the spinal dorsal horn, as well as TRPV1, CD11B, and c-Fos proteins in the hypothalamus of the capsazepine group were lower than those in the mild moxibustion group (<i>P</i>&lt;0.05).</p> Conclusion <p>Mild moxibustion at Shenque (CV8) and Guanyuan (CV4) may alleviate the central pain sensitization in PD rats by down-regulating TRPV1 channel expression in the spinal dorsal horn and hypothalamus, thus playing an analgesic effect.</p>

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Effect of moxibustion at Shenque (CV8) and Guanyuan (CV4) on TRPV1 channel in spinal dorsal horn and hypothalamus of dysmenorrhea rats

  • 期刊论文

  • 发表日期：

    2025年4月23日

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  • Yanqiu Sun,
  • Yulei Liang,
  • Di Wang,
  • Zhiguo Zhao,
  • Liyun Yang,
  • Xuanping Zhang,
  • Yan Zhang,
  • Xuan Zhang,
  • Yanxue Xing,
  • Min Zhou,
  • Xiaoyan Wang,
  • Xinhua Li

摘要

Objective

To investigate the effect of mild moxibustion on transient receptor potential vanilloid type 1 (TRPV1) channel expression in primary dysmenorrhea (PD) rats and explore its mechanism in alleviating central pain sensitization.

Methods

Thirty-two female non-pregnant Wistar rats were randomized into a blank group, a model group, a mild moxibustion group, and a capsazepine group, with 8 rats in each group. Except for the blank group, the other three groups used estradiol benzoate, ice-water bath, and oxytocin to establish the rat PD model of cold-dampness stagnation pattern. The interventions began on day 1 of modeling, once a day, and lasted 10 d. The mild moxibustion group received mild moxibustion at Shenque (CV8) and Guanyuan (CV4), 20 min/time; in the capsazepine group, capsazepine was injected at a dose of 2 mg/(kg·bw). The abdominal pain threshold was measured 10–30 min after oxytocin injection on day 11; enzyme-linked immunosorbent assay was used to detect serum prostaglandin F2α (PGF2α) level; the expression of TRPV1, cluster of differentiation 11B (CD11B), and proto-oncogene c-Fos in the spinal dorsal horn and hypothalamus was detected by immunofluorescence and Western blotting.

Results

Compared to the blank group, the model group showed a decreased pain threshold (P<0.05) and an increased serum PGF2α level with elevated TRPV1, CD11B, and c-Fos protein expression in the spinal dorsal horn and hypothalamus (P<0.05). Compared to the model group, both the mild moxibustion group and capsazepine group showed significantly increased pain thresholds (P<0.05), along with decreased serum PGF2α levels and reduced protein expression levels of TRPV1, CD11B, and c-Fos in the spinal dorsal horn and hypothalamus (P<0.05). Rat pain threshold in the capsazepine group was higher than that in the mild moxibustion group (P<0.05). Serum PGF2α level, the expression levels of CD11B and c-Fos proteins in the spinal dorsal horn, as well as TRPV1, CD11B, and c-Fos proteins in the hypothalamus of the capsazepine group were lower than those in the mild moxibustion group (P<0.05).

Conclusion

Mild moxibustion at Shenque (CV8) and Guanyuan (CV4) may alleviate the central pain sensitization in PD rats by down-regulating TRPV1 channel expression in the spinal dorsal horn and hypothalamus, thus playing an analgesic effect.