<p>Robotic-assisted surgery (RAS) is transforming surgical practice globally; however, its integration in Jordan remains limited. This study aimed to evaluate the knowledge, attitudes, perceptions, barriers and ethical concerns of Jordanian medical students regarding RAS, given its growing relevance in surgical education and practice, and identify relevant gaps to future integration. A national cross-sectional study was conducted among medical students from six Jordanian universities using a structured self-administered online questionnaire. The questionnaire assessed knowledge, attitudes, perceptions, barriers, and ethical concerns related to RAS. Data were analysed using descriptive and chi-square tests, with P &lt; 0.05 considered statistically significant. Subgroup analyses were performed by academic stage (preclinical vs. clinical years) and future career preference (surgical vs. non-surgical). Among 840 participants, 62.6% were females, 22.3% reported prior knowledge of RAS, while 45.8% had no prior knowledge but expressed interest in learning. Only 25.5% were aware of at least one robotic surgical system, and 51.4% correctly defined RAS. Most recognised at least two RAS advantages (76.2%), but fewer identified minimally invasive surgery as a key advance (43.2%). Students interested in surgical specialities showed higher prior RAS knowledge (25.6% vs. 18.8%), and stronger interest in RAS training (80.9% vs. 67.7%) compared with non-surgical peers (P &lt; 0.05). Jordanian medical students showed limited knowledge but generally positive attitudes toward RAS, with a strong interest in receiving formal exposure and training. Despite recognising the benefits of RAS, students also reported notable concerns and identified substantial barriers to its implementation in Jordan, particularly cost, lack of equipment, and insufficiently trained faculty. These findings highlight a clear educational gap and underscore the need to strengthen awareness and structured learning opportunities.</p>