Scholarlink

<p>The rapid emergence of multidrug-resistant bacteria represents a major global health concern, increasing the urgent need to develop novel and efficient antimicrobial strategies. In this work, we report the biosynthesis of silver nanoparticles (AgNPs) using the extract from <i>Anoda cristata (A. cristata)</i> as both a reducing and capping agent. Structural and morphological characterization by X-ray diffraction, UV-Vis spectroscopy, and scanning electron microscopy (SEM) confirmed the formation of spherical silver nanostructures with an average size of 10&#xa0;nm. Fourier-transform infrared spectroscopy (FTIR) suggests the presence of two important flavonoids, acacetin and diosmetin, on the nanoparticles’ surface, which are strongly associated with the observed antimicrobial activity of the nanocomposite. The bactericidal effect of these nanoparticles was evaluated against multidrug-resistant Gram-negative strains, including <i>Escherichia coli</i> (<i>E. coli</i>), <i>Citrobacter</i> spp., and two ESKAPE strains of <i>Pseudomonas aeruginosa</i> (<i>P. aeruginosa</i>), using disk diffusion, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and time-kill assays. The results demonstrate that <i>E. coli</i> displayed higher susceptibility in diffusion tests, whereas MIC and MBC values showed species-dependent responses. Time-kill assays show the impact of bacterial phenotype on antimicrobial dynamics, with complete bacterial inhibition achieved within 6&#xa0;h at MBC conditions. These findings support the potential of AgNPs derived from <i>A. cristata</i> extract as an efficient antimicrobial platform against multidrug-resistant pathogens.</p> Graphical abstract

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Synthesis of phytochemical-capped silver nanoparticles using Anoda cristata extract and their activity against multidrug-resistant Gram-Negative bacteria

  • 期刊论文

  • 发表日期：

    2026年7月14日

查看全文
  • Abigail C. De la Luz,
  • Samantha C. Arellano,
  • L. San Román-Escudero,
  • N. R. Silva-González,
  • Norma E. Rojas-Ruiz,
  • U. Salazar-Kuri,
  • Nery M. Aguilar

摘要

The rapid emergence of multidrug-resistant bacteria represents a major global health concern, increasing the urgent need to develop novel and efficient antimicrobial strategies. In this work, we report the biosynthesis of silver nanoparticles (AgNPs) using the extract from Anoda cristata (A. cristata) as both a reducing and capping agent. Structural and morphological characterization by X-ray diffraction, UV-Vis spectroscopy, and scanning electron microscopy (SEM) confirmed the formation of spherical silver nanostructures with an average size of 10 nm. Fourier-transform infrared spectroscopy (FTIR) suggests the presence of two important flavonoids, acacetin and diosmetin, on the nanoparticles’ surface, which are strongly associated with the observed antimicrobial activity of the nanocomposite. The bactericidal effect of these nanoparticles was evaluated against multidrug-resistant Gram-negative strains, including Escherichia coli (E. coli), Citrobacter spp., and two ESKAPE strains of Pseudomonas aeruginosa (P. aeruginosa), using disk diffusion, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and time-kill assays. The results demonstrate that E. coli displayed higher susceptibility in diffusion tests, whereas MIC and MBC values showed species-dependent responses. Time-kill assays show the impact of bacterial phenotype on antimicrobial dynamics, with complete bacterial inhibition achieved within 6 h at MBC conditions. These findings support the potential of AgNPs derived from A. cristata extract as an efficient antimicrobial platform against multidrug-resistant pathogens.

Graphical abstract