<p>The biodiesel-based research is increasing day-by-day with the available resources including the waste materials to meet the future demand. One of the sustainable waste sources is cooked chicken waste like bones and some flesh parts etc., from food stalls and hotels after serving to their customers. Chicken biriyani is a major familiar and favorite food worldwide. This investigation focuses on using such cooked chicken wastes to produce alternate fuel for diesel engines. The biodiesel was produced from such waste by pyrolysis process then they transesterified to produce biodiesel. This biodiesel quality was improved by blending 25% with diesel (80%) and further enhanced with three different nanoparticles such as silicon dioxide nanoparticles, MWCNT, Copper oxide, and ternary nano fuel (which combines these three nanoparticles in equal proportion in that blend). These nano fuels were tested in a 5.2&#xa0;kW, CI engine test rig with different load conditions. Compared to individual nano fuels, this ternary nano fuel showed improved performance at full load conditions as well as in terms of average performance. Though the performance of nano fuels was found better than base fuel, the results revealed that the ternary nano fuel was outperformed in terms of 3.67% higher BTE, 23.92% reduced CO, 18.34% reduced HC, 8.96% reduced NOx, and 19.62% reduced smoke emission than diesel. Therefore, ternary nano fuel will be the best alternative for diesel engines to operate with improved performance with reduced emissions without any modification in the diesel engines.</p>