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<p>Dengue is a significant global health concern, necessitating rapid, sensitive, and cost-effective tools for early detection. Herein, we report the first label-free electrochemical aptasensor specifically targeting the dengue virus envelope (E) protein, addressing a critical gap in dengue diagnostics beyond conventional NS1-based assays. The aptasensor was developed on a gold electrode through a rational layer-by-layer assembly of a cysteamine self-assembled monolayer, gold nanoparticles (AuNPs), and a thiolated DNA aptamer with high affinity toward the dengue E protein. The aptasensor demonstrates an impressive limit of detection (LOD) of 66&#xa0;fg mL<sup>− 1</sup> within a broad dynamic range of 100&#xa0;fg mL<sup>− 1</sup>–5 ng mL<sup>− 1</sup>, suitable for early-stage detection. Atomic force microscopy (AFM) and electrochemical analysis confirmed successful surface functionalization and target interactions. The aptasensor also showed excellent selectivity against potential interferents, high reproducibility, and long-term stability. Importantly, its analytical performance was validated using dengue-positive human serum samples, showing strong agreement with standard ELISA results. Owing to its first-of-its-kind E-protein targeting strategy, label-free detection, rapid response, and exceptional sensitivity, the proposed aptasensor represents a promising and cost-effective point-of-care diagnostic tool for early-stage dengue detection, particularly suited for resource-limited settings.</p>

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Gold nanoparticle-enhanced electrochemical aptasensor for ultrasensitive detection of dengue E protein

  • 期刊论文

  • 发表日期：

    2026年4月28日

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  • Kokilavani R,
  • Hiranmoy Kotal,
  • Thangapandi Kalyani,
  • Arunima Lala,
  • Biraj Bhuyan,
  • Saikat Kumar Jana

摘要

Dengue is a significant global health concern, necessitating rapid, sensitive, and cost-effective tools for early detection. Herein, we report the first label-free electrochemical aptasensor specifically targeting the dengue virus envelope (E) protein, addressing a critical gap in dengue diagnostics beyond conventional NS1-based assays. The aptasensor was developed on a gold electrode through a rational layer-by-layer assembly of a cysteamine self-assembled monolayer, gold nanoparticles (AuNPs), and a thiolated DNA aptamer with high affinity toward the dengue E protein. The aptasensor demonstrates an impressive limit of detection (LOD) of 66 fg mL− 1 within a broad dynamic range of 100 fg mL− 1–5 ng mL− 1, suitable for early-stage detection. Atomic force microscopy (AFM) and electrochemical analysis confirmed successful surface functionalization and target interactions. The aptasensor also showed excellent selectivity against potential interferents, high reproducibility, and long-term stability. Importantly, its analytical performance was validated using dengue-positive human serum samples, showing strong agreement with standard ELISA results. Owing to its first-of-its-kind E-protein targeting strategy, label-free detection, rapid response, and exceptional sensitivity, the proposed aptasensor represents a promising and cost-effective point-of-care diagnostic tool for early-stage dengue detection, particularly suited for resource-limited settings.