Scholarlink

<p>Ofloxacin (OFL), as third-generation fluoroquinolone antibiotics, is commonly used to treat bacterial infections in humans and animals. However, excessive use of OFL has led to significant environmental contamination, raising concerns about food safety and public health. The current detection methods for OFL residues often come with lengthy detection times and narrow detection ranges. In this context, we developed a fluorescence aptasensor based on FRET (Förster Resonance Energy Transfer) between the FAM fluorescent group and gold nanoparticles (AuNPs), offering a more rapid and a wide detection range, as well as achieving the detection of OFL in multiple practical samples. The nucleic acid aptamer labeled with FAM (FAM-Apt) binds with the AuNPs to quench FAM fluorescence due to FRET. Upon addition of OFL, it preferentially binds to aptamers, leading to an increase in the fluorescence intensity of FAM-Apt. Therefore, this strategy provided the possibility for OFL detection. The results show that this sensor was able to selectively detect OFL with a linear range of 0–1500&#xa0;nM. The limit of detection was 4.84&#xa0;nM under optimized conditions. The aptasensor successfully detected OFL in serum, urine, tap water, river water, pork, chicken and milk, with spiked recoveries ranging from 98.00 to 102.56% and relative standard deviations ranging from 0.41 to 6.99%, demonstrating its potential for food safety applications. Overall, this research offers a wide detection range, faster, and more affordable method for OFL detection in practical samples.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

A fluorescent aptasensor based on fluorescence resonance energy transfer for the detection of ofloxacin

  • 期刊论文

  • 发表日期：

    2025年4月10日

查看全文
  • Wei-Chuang Kong,
  • Miao-Miao Wu,
  • Chen-Chen Li,
  • Jiang-Nan Wang,
  • Ya-Li Meng,
  • Xiao-Juan Jia,
  • Zhen-Hui Xin,
  • Yan-Fei Kang

摘要

Ofloxacin (OFL), as third-generation fluoroquinolone antibiotics, is commonly used to treat bacterial infections in humans and animals. However, excessive use of OFL has led to significant environmental contamination, raising concerns about food safety and public health. The current detection methods for OFL residues often come with lengthy detection times and narrow detection ranges. In this context, we developed a fluorescence aptasensor based on FRET (Förster Resonance Energy Transfer) between the FAM fluorescent group and gold nanoparticles (AuNPs), offering a more rapid and a wide detection range, as well as achieving the detection of OFL in multiple practical samples. The nucleic acid aptamer labeled with FAM (FAM-Apt) binds with the AuNPs to quench FAM fluorescence due to FRET. Upon addition of OFL, it preferentially binds to aptamers, leading to an increase in the fluorescence intensity of FAM-Apt. Therefore, this strategy provided the possibility for OFL detection. The results show that this sensor was able to selectively detect OFL with a linear range of 0–1500 nM. The limit of detection was 4.84 nM under optimized conditions. The aptasensor successfully detected OFL in serum, urine, tap water, river water, pork, chicken and milk, with spiked recoveries ranging from 98.00 to 102.56% and relative standard deviations ranging from 0.41 to 6.99%, demonstrating its potential for food safety applications. Overall, this research offers a wide detection range, faster, and more affordable method for OFL detection in practical samples.