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<p>Black carrot pomace (BCP), a byproduct of juice industry, is rich in bioactive compounds. This study employed deep eutectic solvents (DES) combined with ultrasound-assisted extraction (UAE) to maximize the extraction of bioactive compounds from BCP. Four DES were evaluated. The optimum conditions were determined using response surface methodology (RSM) and validated. DES 1 showing the highest extraction efficiency for total monomeric anthocyanin content (TMAC) of 0.056 ± 0.001&#xa0;mg C-3-GE/g with predicted value of 0.055&#xa0;mg C-3-GE/g, DES 2 for total carotenoid content (TCC) of 5.021 ± 0.08&#xa0;mg B-CE/g with predicted value of 4.926&#xa0;mg B-CE/g and DES 4 for both total phenolic content (TPC) of 25.782 ± 0.35&#xa0;mg GAE/g with predicted value of 26.429&#xa0;mg GAE/g and total flavonoid content (TFC) of 20.093 ± 0.49&#xa0;mg QE/g with predicted value of 20.161&#xa0;mg QE/g. Among all extracts, highest TPC containing extract prepared in DES 4 exhibited maximum antioxidant activity. The highest storage stability of TMAC was observed at 4 ℃ in the dark. This study provides a high-efficiency, eco-friendly alternative to conventional solvents, while maintaining antioxidant activity of the extracted compounds.</p>

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Optimization of bioactive compounds from black carrot pomace using deep eutectic solvents: Box-Behnken design, validation, antioxidant and stability studies

  • 期刊论文

  • 发表日期：

    2026年5月2日

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  • Jaspreet Kaur,
  • Ramandeep Kaur,
  • Urvashi Bhardwaj,
  • Arashdeep Singh

摘要

Black carrot pomace (BCP), a byproduct of juice industry, is rich in bioactive compounds. This study employed deep eutectic solvents (DES) combined with ultrasound-assisted extraction (UAE) to maximize the extraction of bioactive compounds from BCP. Four DES were evaluated. The optimum conditions were determined using response surface methodology (RSM) and validated. DES 1 showing the highest extraction efficiency for total monomeric anthocyanin content (TMAC) of 0.056 ± 0.001 mg C-3-GE/g with predicted value of 0.055 mg C-3-GE/g, DES 2 for total carotenoid content (TCC) of 5.021 ± 0.08 mg B-CE/g with predicted value of 4.926 mg B-CE/g and DES 4 for both total phenolic content (TPC) of 25.782 ± 0.35 mg GAE/g with predicted value of 26.429 mg GAE/g and total flavonoid content (TFC) of 20.093 ± 0.49 mg QE/g with predicted value of 20.161 mg QE/g. Among all extracts, highest TPC containing extract prepared in DES 4 exhibited maximum antioxidant activity. The highest storage stability of TMAC was observed at 4 ℃ in the dark. This study provides a high-efficiency, eco-friendly alternative to conventional solvents, while maintaining antioxidant activity of the extracted compounds.