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<p>A method was developed for the simultaneous determination of bongkrekic acid (BA), isobongkrekic acid (IBA), and toxoflavin (TF) in fermented corn noodle. The analytes were extracted using acidified acetonitrile–water (80:20, v/v), purified by the QuEChERS method, and analyzed by ultra-performance liquid chromatography–tandem mass spectrometry, with quantification using matrix-matched calibration. BA and IBA showed good linearity in the range of 0.4–20 ng mL<InlineEquation ID="IEq1"> <EquationSource Format="TEX">\({^1}\)</EquationSource> </InlineEquation>, while TF exhibited linearity between 0.2–10 ng mL<InlineEquation ID="IEq2"> <EquationSource Format="TEX">\({^1}\)</EquationSource> </InlineEquation>. At three spiked concentration levels, the coefficients of determination (r<InlineEquation ID="IEq3"> <EquationSource Format="TEX">\({^2}\)</EquationSource> </InlineEquation>) exceeded 0.99. Average recoveries ranged from 89.5% to 98.7% for BA, 91.0% to 101.9% for IBA, and 90.2% to 98.8% for TF, with relative standard deviations of 0.3% to 1.5%, 1.2 to 2.2%, and 0.7 to 2.1%, respectively. Compared with traditional hydrophilic–lipophilic balance solid-phase extraction, this method eliminates the need for equilibrium activation, wetting, and elution steps, reducing cleanup time and organic solvent consumption. This straightforward, sensitive, and efficient approach is suitable for routine analysis of BA, IBA, and TF in bulk corn noodle products.</p>

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Determination of three types of toxins produced by Burkholderia gladioli pv. cocovenenans. in fermented corn noodles using QuEChERS purification and UPLC-MS

  • 期刊论文

  • 发表日期：

    2026年5月19日

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  • Chang Bo,
  • Yang Shuixian,
  • Gao Bin,
  • Gao Huihua,
  • Hou Chanxia,
  • Zhou Yu,
  • Cao Wenjun,
  • Zhao Hongyang,
  • Zhu Jinyan,
  • Chan Nan

摘要

A method was developed for the simultaneous determination of bongkrekic acid (BA), isobongkrekic acid (IBA), and toxoflavin (TF) in fermented corn noodle. The analytes were extracted using acidified acetonitrile–water (80:20, v/v), purified by the QuEChERS method, and analyzed by ultra-performance liquid chromatography–tandem mass spectrometry, with quantification using matrix-matched calibration. BA and IBA showed good linearity in the range of 0.4–20 ng mL \({^1}\) , while TF exhibited linearity between 0.2–10 ng mL \({^1}\) . At three spiked concentration levels, the coefficients of determination (r \({^2}\) ) exceeded 0.99. Average recoveries ranged from 89.5% to 98.7% for BA, 91.0% to 101.9% for IBA, and 90.2% to 98.8% for TF, with relative standard deviations of 0.3% to 1.5%, 1.2 to 2.2%, and 0.7 to 2.1%, respectively. Compared with traditional hydrophilic–lipophilic balance solid-phase extraction, this method eliminates the need for equilibrium activation, wetting, and elution steps, reducing cleanup time and organic solvent consumption. This straightforward, sensitive, and efficient approach is suitable for routine analysis of BA, IBA, and TF in bulk corn noodle products.