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<p>Magnesium oxide nanoparticles (MgO NPs) were biofabricated by using <i>Murraya koenigii</i> (curry plant) leaves essential oil as a reducing and capping agent and tested for their antibacterial and antioxidant potential. Curry leaves essential oil (CLEO) was extracted by hydrodistillation using a Clevenger-type apparatus. Gas Chromatography-Mass Spectrometry analysis of CLEO reported the presence of α-pinene (32.40%) and β-phellandrene (21.93%) as major constituents. The MgO NPs were prepared by using CLEO via sol-gel method and their zeta potential was + 17.3 mV and polydispersity index was 0.191. UV-visible spectrum of MgO NPs showed absorption band at 289&#xa0;nm. Fourier Transform-Infrared spectrum of MgO NPs revealed functionalisation of nanoparticles with CLEO. X-Ray Diffraction diffractogram depicted an average crystallite size of 11.16&#xa0;nm with a face-centered cubic crystal lattice. Energy Dispersive X-ray spectrum showed peaks of Mg, O and C elements. Transmission Electron Microscopy micrographs showed them as aggregated spherical nanoparticles with diameters ranging from 7.51 to 22.54&#xa0;nm. MgO NPs exhibited higher antibacterial potency than CLEO having lesser values of minimum inhibitory concentration against <i>Escherichia coli</i>,<i> Pseudomonas aeruginosa</i>,<i> Staphylococcus aureus</i> and <i>Bacillus subtilis</i>, with both exhibiting bacteriostatic activity as revealed by time-kill kinetics assay. The NPs also exhibited lower IC<sub>50</sub> value than CLEO in 2, 2-diphenyl-1-picrylhydrazyl and nitric oxide free radical scavenging assay. In mustard oil degradation assay, MgO NPs exhibited less increase in peroxide value, anisidine value, thiobarbituric acid value and total oxidation value than CLEO. NPs were safe for administration upto 1000&#xa0;µg/mL as evaluated by hemocompatibility assay and were cytotoxic against HT-29 cell line (IC<sub>50</sub> value 989.70&#xa0;µg/mL). Hence, MgO NPs fabricated by using CLEO may be used as a biopreservative as a substitute for synthetic analogue as an approach towards sustainable development.</p>

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Biofabrication of magnesium oxide nanoparticles with Murraya koenigii essential oil with enhanced antibacterial and antioxidant potential

  • 期刊论文

  • 发表日期：

    2026年3月24日

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  • Manveen Kaur Saggu,
  • Sonia Kaushal,
  • Pratibha Vyas

摘要

Magnesium oxide nanoparticles (MgO NPs) were biofabricated by using Murraya koenigii (curry plant) leaves essential oil as a reducing and capping agent and tested for their antibacterial and antioxidant potential. Curry leaves essential oil (CLEO) was extracted by hydrodistillation using a Clevenger-type apparatus. Gas Chromatography-Mass Spectrometry analysis of CLEO reported the presence of α-pinene (32.40%) and β-phellandrene (21.93%) as major constituents. The MgO NPs were prepared by using CLEO via sol-gel method and their zeta potential was + 17.3 mV and polydispersity index was 0.191. UV-visible spectrum of MgO NPs showed absorption band at 289 nm. Fourier Transform-Infrared spectrum of MgO NPs revealed functionalisation of nanoparticles with CLEO. X-Ray Diffraction diffractogram depicted an average crystallite size of 11.16 nm with a face-centered cubic crystal lattice. Energy Dispersive X-ray spectrum showed peaks of Mg, O and C elements. Transmission Electron Microscopy micrographs showed them as aggregated spherical nanoparticles with diameters ranging from 7.51 to 22.54 nm. MgO NPs exhibited higher antibacterial potency than CLEO having lesser values of minimum inhibitory concentration against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis, with both exhibiting bacteriostatic activity as revealed by time-kill kinetics assay. The NPs also exhibited lower IC50 value than CLEO in 2, 2-diphenyl-1-picrylhydrazyl and nitric oxide free radical scavenging assay. In mustard oil degradation assay, MgO NPs exhibited less increase in peroxide value, anisidine value, thiobarbituric acid value and total oxidation value than CLEO. NPs were safe for administration upto 1000 µg/mL as evaluated by hemocompatibility assay and were cytotoxic against HT-29 cell line (IC50 value 989.70 µg/mL). Hence, MgO NPs fabricated by using CLEO may be used as a biopreservative as a substitute for synthetic analogue as an approach towards sustainable development.