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<p>In the herein study, we put forward the hydrothermal synthesis of intrinsic dual-emission carbon dots (d Cu, N-CDs) utilizing copper gluconate, 2,5-diaminobenzenesulfonic acid, and o-phenylenediamine as raw materials, with nitric acid as a passivating agent and ultra-pure water as the solvent. The synthesis parameters were optimized to achieve d Cu, N-CDs with dual fluorescence emission peaks at 425&#xa0;nm (blue) and 535&#xa0;nm (yellow), which exhibited outstanding water solubility, steady fluorescence properties, and a broad range of surface functional groups. d Cu, N-CDs demonstrated a highly sensitive fluorescence response to Auramine O (AO) through a combination of dynamic quenching and energy transfer mechanisms. The method enabled accurate detection of AO across a wide concentration range (0.1–10 µmol/L, 10–65 µmol/L) with a detection limit of 0.06 µmol/L. Besides, the selectivity and anti-interference ability of d Cu, N-CDs were validated through interference experiments with various substances, showing no significant interference with AO detection. The probe’s effectiveness was further demonstrated in real-world applications by detecting AO in traditional Chinese medicine samples such as <i>Astragalus membranaceus</i> and <i>Phellodendron amurense</i>, with high recovery rates. The results underscore the potential of d Cu, N-CDs as a dual-emission ratiometric fluorescence probe for the sensitive and reliable detection of AO in complex matrices. This study opens new option for the development of advanced fluorescent sensors, with significant potential for applications in food safety, pharmaceutical testing, environmental monitoring.</p>

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Intrinsic dual-emission carbon dots for sensitive and selective detection of auramine O

  • 期刊论文

  • 发表日期：

    2026年1月23日

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  • Jinzhi Yu,
  • Chao Luo,
  • Haiyan Qi,
  • Jun Li,
  • Yunpeng Zhao,
  • Rui Jie,
  • Chenyu Qi

摘要

In the herein study, we put forward the hydrothermal synthesis of intrinsic dual-emission carbon dots (d Cu, N-CDs) utilizing copper gluconate, 2,5-diaminobenzenesulfonic acid, and o-phenylenediamine as raw materials, with nitric acid as a passivating agent and ultra-pure water as the solvent. The synthesis parameters were optimized to achieve d Cu, N-CDs with dual fluorescence emission peaks at 425 nm (blue) and 535 nm (yellow), which exhibited outstanding water solubility, steady fluorescence properties, and a broad range of surface functional groups. d Cu, N-CDs demonstrated a highly sensitive fluorescence response to Auramine O (AO) through a combination of dynamic quenching and energy transfer mechanisms. The method enabled accurate detection of AO across a wide concentration range (0.1–10 µmol/L, 10–65 µmol/L) with a detection limit of 0.06 µmol/L. Besides, the selectivity and anti-interference ability of d Cu, N-CDs were validated through interference experiments with various substances, showing no significant interference with AO detection. The probe’s effectiveness was further demonstrated in real-world applications by detecting AO in traditional Chinese medicine samples such as Astragalus membranaceus and Phellodendron amurense, with high recovery rates. The results underscore the potential of d Cu, N-CDs as a dual-emission ratiometric fluorescence probe for the sensitive and reliable detection of AO in complex matrices. This study opens new option for the development of advanced fluorescent sensors, with significant potential for applications in food safety, pharmaceutical testing, environmental monitoring.