Scholarlink

<p><i>Vaccinium vitis-idaea</i> L. is a nutrient-dense functional food that is rich in a variety of bioactive constituents. Microwave-assisted ethanol extraction was developed to extract the active components from <i>Vaccinium vitis-idaea</i> L. fruit powder. A total of 29 compounds were identified by UPLC-MS/MS combined with Compound Discoverer 3.3 software, which included phenolic acids, anthocyanins, flavonoids, and triterpenoids. Furthermore, UPLC-MS/MS quantitative analysis methods of oleanolic acid, ferulic acid, chlorogenic acid, Cyanidin-3-glucoside (kuromanin), α-linolenic acid and docosanoic acid were established (R² &gt;0.999, recoveries 95–101%), and the determined results as 4.09 ± 0.09&#xa0;mg/g, 0.86 ± 0.08&#xa0;mg/g, 34.91 ± 0.50&#xa0;mg/g, 5.01 ± 0.16&#xa0;mg/g, 0.34 ± 0.02&#xa0;mg/g, 0.68 ± 0.07&#xa0;mg/g, respectively. Compared with traditional methods, this method has shorter detection time and higher sensitivity. This study provides a quantitative basis for standardizing future analytical and functional studies on <i>Vaccinium vitis-idaea</i> L. while highlighting chlorogenic acid and kuromanin to lay the foundation for subsequent studies on antioxidant and health-promoting mechanisms.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Identification and determination of anthocyanins and phenolic acids in Vaccinium vitis-idaea L. by UPLC-MS/MS

  • 期刊论文

  • 发表日期：

    2025年11月21日

查看全文
  • Liying Cui,
  • Shengkai Liu,
  • Siqi He,
  • Hongji Wang,
  • Guangru Chu,
  • Aijiao Wang,
  • Yujie Fu,
  • Zhiguo Liu

摘要

Vaccinium vitis-idaea L. is a nutrient-dense functional food that is rich in a variety of bioactive constituents. Microwave-assisted ethanol extraction was developed to extract the active components from Vaccinium vitis-idaea L. fruit powder. A total of 29 compounds were identified by UPLC-MS/MS combined with Compound Discoverer 3.3 software, which included phenolic acids, anthocyanins, flavonoids, and triterpenoids. Furthermore, UPLC-MS/MS quantitative analysis methods of oleanolic acid, ferulic acid, chlorogenic acid, Cyanidin-3-glucoside (kuromanin), α-linolenic acid and docosanoic acid were established (R² >0.999, recoveries 95–101%), and the determined results as 4.09 ± 0.09 mg/g, 0.86 ± 0.08 mg/g, 34.91 ± 0.50 mg/g, 5.01 ± 0.16 mg/g, 0.34 ± 0.02 mg/g, 0.68 ± 0.07 mg/g, respectively. Compared with traditional methods, this method has shorter detection time and higher sensitivity. This study provides a quantitative basis for standardizing future analytical and functional studies on Vaccinium vitis-idaea L. while highlighting chlorogenic acid and kuromanin to lay the foundation for subsequent studies on antioxidant and health-promoting mechanisms.