Synergisticm with thyme essential oil and ε-polylysine: an innovative study for cold meat preservation active packaging film
摘要
Active packaging represents a cutting-edge frontier in food preservation, attracting significant attention. However, the effectiveness of packaging films containing single active substances remains limited. In this study, a novel synergistic packaging film was developed using basil seed gum (BSG) and sodium carboxymethyl cellulose (CMC) as the film matrix, and thyme essential oil (TEO) and ε-polylysine (PL) as dual active components. This combination leveraged the complementary antimicrobial mechanisms of TEO and PL, enhancing film functionality. Comprehensive characterization revealed that the TEO/PL composite film exhibited a denser microstructure, superior mechanical properties (18.42 ± 0.33 MPa tensile strength, 5.77 ± 0.09% elongation at break), and significantly reduced UV transmittance, water vapor permeability ((5.22 ± 0.59) × 10⁻¹¹ g mm/m² s Pa), and oxygen permeability ((4.85 ± 0.28) × 10⁻¹⁰ g/m² s). FT-IR analysis demonstrated excellent compatibility between the components. Notably, the composite film achieved remarkable antibacterial performance against Escherichia coli with an inhibition zone diameter of 11.54 ± 0.23 mm, outperforming films containing TEO or PL alone. In cold meat preservation trials, the composite film extended the storage life to 9 days, as evidenced by lower microbial counts, TBARS values, and TVB-N levels compared to control groups. This work introduces a synergistic approach to active packaging by combining natural actives with a renewable biopolymer matrix, providing a sustainable and high-performance solution for cold meat preservation. The findings highlight the potential of dual-active films to overcome limitations of single-active designs, paving the way for advanced food packaging technologies.