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<p>Changes in the pharmacological properties of natural products may depend on the cooking processes; therefore, <i>Pleurotus ostreatus</i> was immediately subjected to moist heat cooking. Molecular characterization identified <i>P. ostreatus</i> with accession number PQ429007. High-performance liquid chromatography (HPLC) revealed that the extract of uncooked <i>P. ostreatus</i> (ECPS) contained high concentrations of catechin (75,556.07 µg/g), gallic acid (66,183.14 µg/g), naringenin (50,021.85 µg/g), hesperetin (22.78 µg/g), and quercetin (68.54 µg/g). In contrast, the concentrations in the extract of cooked <i>P. ostreatus</i> (EUCPS) decreased to 52,212.40, 51,212.19, 34,238.17, 2.67, 27.67, 16,598.19, and 9778.36 µg/g, respectively. ECPS demonstrated&#xa0;greater efficacy than EUCPS when tested on normal WI38 cells, HeLa, and PC3 cancer cells. Both extracts showed low toxicity to normal WI38 cells, with IC<sub>50</sub> values of 193.42 ± 1.54 µg/mL for ECPS and 187.69 ± 2.81 µg/mL for EUCPS. In comparison, IC<sub>50</sub> values for PC3 cells were 107.4 ± 2.57 µg/mL for ECPS and 143.91 ± 1.7 µg/mL for EUCPS, while HeLa cells showed IC<sub>50</sub> values of 109.71 ± 0.22 µg/mL for ECPS and 154.17 ± 1.93 µg/mL for EUCPS. Both ECPS and EUCPS exhibited antioxidant activity, with IC<sub>50</sub> values of 16.17 µg/mL and 49.31 µg/mL, respectively. The anti-obesity activity provides IC<sub>50</sub> values of 25.84 µg/mL for ECPS, 37.13 µg/mL for EUCPS, and 6.93 µg/mL for orlistat. Molecular docking studies demonstrated strong binding affinities with catechin exhibiting scores of − 6.3204 and − 6.20723 kcal/mol against PC3 PDB ID: 2Q7L and 3PE6, respectively. Gallic acid displayed binding scores of − 5.27521 and − 4.81822 kcal/mol, respectively.</p>

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Chemical characterization, anticancer, antioxidant and anti-obesity activities with molecular docking studies of Pleurotus ostreatus biomass exposed to moist heat

  • 期刊论文

  • 发表日期：

    2025年2月26日

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  • Naif K. Binsaleh,
  • Abdulrahman S. Bazaid,
  • Heba Barnawi,
  • Bandar Alharbi,
  • Ahmed Alsolami,
  • Albatool Y. Babsail,
  • Samy Selim,
  • Tarek M. Abdelghany,
  • Reham M. Elbaz,
  • Husam Qanash

摘要

Changes in the pharmacological properties of natural products may depend on the cooking processes; therefore, Pleurotus ostreatus was immediately subjected to moist heat cooking. Molecular characterization identified P. ostreatus with accession number PQ429007. High-performance liquid chromatography (HPLC) revealed that the extract of uncooked P. ostreatus (ECPS) contained high concentrations of catechin (75,556.07 µg/g), gallic acid (66,183.14 µg/g), naringenin (50,021.85 µg/g), hesperetin (22.78 µg/g), and quercetin (68.54 µg/g). In contrast, the concentrations in the extract of cooked P. ostreatus (EUCPS) decreased to 52,212.40, 51,212.19, 34,238.17, 2.67, 27.67, 16,598.19, and 9778.36 µg/g, respectively. ECPS demonstrated greater efficacy than EUCPS when tested on normal WI38 cells, HeLa, and PC3 cancer cells. Both extracts showed low toxicity to normal WI38 cells, with IC50 values of 193.42 ± 1.54 µg/mL for ECPS and 187.69 ± 2.81 µg/mL for EUCPS. In comparison, IC50 values for PC3 cells were 107.4 ± 2.57 µg/mL for ECPS and 143.91 ± 1.7 µg/mL for EUCPS, while HeLa cells showed IC50 values of 109.71 ± 0.22 µg/mL for ECPS and 154.17 ± 1.93 µg/mL for EUCPS. Both ECPS and EUCPS exhibited antioxidant activity, with IC50 values of 16.17 µg/mL and 49.31 µg/mL, respectively. The anti-obesity activity provides IC50 values of 25.84 µg/mL for ECPS, 37.13 µg/mL for EUCPS, and 6.93 µg/mL for orlistat. Molecular docking studies demonstrated strong binding affinities with catechin exhibiting scores of − 6.3204 and − 6.20723 kcal/mol against PC3 PDB ID: 2Q7L and 3PE6, respectively. Gallic acid displayed binding scores of − 5.27521 and − 4.81822 kcal/mol, respectively.