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Purpose <p>To establish axenic cultivation of a human-derived <i>Breviata anathema</i> (strain T76) isolated from the stool of a patient with fascioliasis and to characterize its biological properties.</p> Methods <p>T76 was maintained in American Type Culture Collection (ATCC) medium 207, supplemented with sulfate-reducing <i>Desulfovibrio desulfuricans</i> and co-cultured with <i>Pseudomonas aeruginosa</i>, at 30&#xa0;°C. Axenic cultivation was attempted by transferring T76 flagellates to various axenic culture media supplemented with antibiotics for parasitic intestinal protozoan species.</p> Results <p>The 18&#xa0;S rRNA gene sequence of T76 exhibited significant homology with the free-living, bacterivorous <i>B</i>. <i>anathema</i> (ATCC 50338), a member of the rare clade Obazoa. Axenic cultivation of <i>B. anathema</i> (T76) was achieved in YIGADHA-S medium, in which gluconic acid and dihydroxyacetone replaced glucose as energy substrates.</p> Conclusion <p><i>B</i>. <i>anathema</i> (T76) was isolated from a patient with fascioliasis, exposed through consumption of aquatic plants from ponds, a known habitat of this organism. Axenic cultivation of T76 was established in YIGADHA-S medium, originally developed for <i>Entamoeba dispar</i>, and cyst-like spherical bodies harboring flagellates were observed and exhibited resistance to 0.1&#xa0;N HCl.</p>

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Axenic Cultivation of the First Human-Derived Breviata anathema from a Patient with Fascioliasis in West Bengal, India

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  • 发表日期：

    2026年7月27日

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  • Sanjib Kumar Sardar,
  • Ajanta Ghosal,
  • Yumiko Saito-Nakano,
  • Jun Suzuki,
  • Sandipan Ganguly,
  • Seiki Kobayashi

摘要

Purpose

To establish axenic cultivation of a human-derived Breviata anathema (strain T76) isolated from the stool of a patient with fascioliasis and to characterize its biological properties.

Methods

T76 was maintained in American Type Culture Collection (ATCC) medium 207, supplemented with sulfate-reducing Desulfovibrio desulfuricans and co-cultured with Pseudomonas aeruginosa, at 30 °C. Axenic cultivation was attempted by transferring T76 flagellates to various axenic culture media supplemented with antibiotics for parasitic intestinal protozoan species.

Results

The 18 S rRNA gene sequence of T76 exhibited significant homology with the free-living, bacterivorous B. anathema (ATCC 50338), a member of the rare clade Obazoa. Axenic cultivation of B. anathema (T76) was achieved in YIGADHA-S medium, in which gluconic acid and dihydroxyacetone replaced glucose as energy substrates.

Conclusion

B. anathema (T76) was isolated from a patient with fascioliasis, exposed through consumption of aquatic plants from ponds, a known habitat of this organism. Axenic cultivation of T76 was established in YIGADHA-S medium, originally developed for Entamoeba dispar, and cyst-like spherical bodies harboring flagellates were observed and exhibited resistance to 0.1 N HCl.