Scholarlink

Purpose <p><i>Plasmodium falciparum</i> (<i>Pf</i>) is the deadliest malaria parasite species, and its genetic diversity is a significant obstacle to its effective control. Here, we analyzed the genetic diversity and phylogeny of <i>Pf</i> isolates collected in Cameroon using merozoite surface proteins 1 and 2 (<i>msp1/2</i>) markers.</p> Methods <p>Samples were collected in three malaria epidemiological facets of three regions of Cameroon (Littoral, North, Far North). The <i>msp1</i> block 2 and <i>msp2</i> block 3 were genotyped using polymerase chain reaction and Sanger sequencing. The structural organization the <i>msp1/2</i> allelic families (K1, MAD20, and RO33 for <i>msp1</i>, IC/3D7, and FC27 for <i>msp2</i>) was analyzed, while their phylogenetic relatedness was assessed in comparison with ~ 2500 good quality sequences from various geographical areas.</p> Results <p>The K1 and MAD20 families had high structural diversity due to the repetition of 3-amino acid repeats (SGT, SGP, and SAQ for K1, SGG, and SVA for MAD20). RO33 sequences presented several mutation points. Tetramer to decamer repetitive amino acids were identified in IC/3D7 sequences mainly GSGA (31.3%) and GASGSA (25%). The 32-aa sequence of the FC27 family showed non-synonymous substitution, insertion, and deletions. The K1 and RO33 sequences were phylogenetically closer to those from China/Myanmar, while MAD20, IC/3D7, and FC27 sequences were closer to those from India, Panama, and Papua New Guinea. The Cameroonian <i>Pfmsp1/2</i> sequences showed a high genetic structure with phylogenetic patterns outlining a complex <i>Pf</i> population structure.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Genetic Diversity and Phylogenetic Relatedness of Cameroonian Plasmodium falciparum Isolates and Comparative Analysis with Global Populations

  • 期刊论文

  • 发表日期：

    2025年7月12日

查看全文
  • Loick P. Kojom Foko,
  • Joseph Hawadak,
  • Vineeta Singh

摘要

Purpose

Plasmodium falciparum (Pf) is the deadliest malaria parasite species, and its genetic diversity is a significant obstacle to its effective control. Here, we analyzed the genetic diversity and phylogeny of Pf isolates collected in Cameroon using merozoite surface proteins 1 and 2 (msp1/2) markers.

Methods

Samples were collected in three malaria epidemiological facets of three regions of Cameroon (Littoral, North, Far North). The msp1 block 2 and msp2 block 3 were genotyped using polymerase chain reaction and Sanger sequencing. The structural organization the msp1/2 allelic families (K1, MAD20, and RO33 for msp1, IC/3D7, and FC27 for msp2) was analyzed, while their phylogenetic relatedness was assessed in comparison with ~ 2500 good quality sequences from various geographical areas.

Results

The K1 and MAD20 families had high structural diversity due to the repetition of 3-amino acid repeats (SGT, SGP, and SAQ for K1, SGG, and SVA for MAD20). RO33 sequences presented several mutation points. Tetramer to decamer repetitive amino acids were identified in IC/3D7 sequences mainly GSGA (31.3%) and GASGSA (25%). The 32-aa sequence of the FC27 family showed non-synonymous substitution, insertion, and deletions. The K1 and RO33 sequences were phylogenetically closer to those from China/Myanmar, while MAD20, IC/3D7, and FC27 sequences were closer to those from India, Panama, and Papua New Guinea. The Cameroonian Pfmsp1/2 sequences showed a high genetic structure with phylogenetic patterns outlining a complex Pf population structure.

Graphical Abstract