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<p>This study employed Illumina HiSeq™ 2000 sequencing to compare spleen transcriptomes of <i>Uronema marinum</i>-infected and healthy <i>Takifugu rubripes</i>. High-throughput analysis generated 25.6 Gb (infected) and 23.39 Gb (control) high-quality reads, meeting stringent quality standards (Q20 ≥ 98.3%, Q30 ≥ 95.1%). DESeq analysis identified 238 differentially expressed genes (116 upregulated/122 downregulated), functionally categorized into Gene Ontology (GO) domains: biological processes (54.03%), molecular functions (31.78%), and cellular components (14.19%). Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment revealed 62 signaling cascades, with 80% of the top 20 pathways involving immune regulation—including cytokine-receptor interactions, focal adhesion, Jak-STAT signaling, and IgA-mediated immunity. Core immune components (<i>CXCR4</i>, <i>CCR9/CCL19</i>, <i>IL6R/IL12RB2</i>) demonstrated coordinated regulation with ECM-RTK complexes and cytoskeletal modulators (<i>RhoGAP</i>, <i>Paxillin</i>). Mechanistically, <i>PI3K-AKT/PKB</i> signaling cascades emerged as central regulators, interacting with <i>SHP2</i> phosphatases and Sprouty inhibitors to orchestrate anti-parasitic responses. Quantitative validation confirmed transcriptomic patterns, revealing &gt; 85% concordance in immune gene expression. As the first comprehensive analysis of teleost immunity against scuticociliates, this work delineates evolutionarily conserved defense mechanisms in marine fish, including three novel regulatory axes: Toll-like receptor signaling for pathogen recognition, complement-mediated lysis cascades, and antigen processing/presentation pathways. These findings identify candidate molecular targets and immune pathway interactions, laying groundwork for future development of targeted anti-parasitic strategies in aquaculture systems.</p>

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Transcriptomic Analysis of Gene Expression in Immune Pathways in the Spleen of Takifugu rubripes After Infection with Scuticociliate Uronema marinum

  • 期刊论文

  • 发表日期：

    2025年6月27日

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  • Qingmeihui Sun,
  • Yanqi Gao,
  • Xiaohang Wang,
  • Gengtong Zhao,
  • Xiuli Wang,
  • Ruijun Li

摘要

This study employed Illumina HiSeq™ 2000 sequencing to compare spleen transcriptomes of Uronema marinum-infected and healthy Takifugu rubripes. High-throughput analysis generated 25.6 Gb (infected) and 23.39 Gb (control) high-quality reads, meeting stringent quality standards (Q20 ≥ 98.3%, Q30 ≥ 95.1%). DESeq analysis identified 238 differentially expressed genes (116 upregulated/122 downregulated), functionally categorized into Gene Ontology (GO) domains: biological processes (54.03%), molecular functions (31.78%), and cellular components (14.19%). Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment revealed 62 signaling cascades, with 80% of the top 20 pathways involving immune regulation—including cytokine-receptor interactions, focal adhesion, Jak-STAT signaling, and IgA-mediated immunity. Core immune components (CXCR4, CCR9/CCL19, IL6R/IL12RB2) demonstrated coordinated regulation with ECM-RTK complexes and cytoskeletal modulators (RhoGAP, Paxillin). Mechanistically, PI3K-AKT/PKB signaling cascades emerged as central regulators, interacting with SHP2 phosphatases and Sprouty inhibitors to orchestrate anti-parasitic responses. Quantitative validation confirmed transcriptomic patterns, revealing > 85% concordance in immune gene expression. As the first comprehensive analysis of teleost immunity against scuticociliates, this work delineates evolutionarily conserved defense mechanisms in marine fish, including three novel regulatory axes: Toll-like receptor signaling for pathogen recognition, complement-mediated lysis cascades, and antigen processing/presentation pathways. These findings identify candidate molecular targets and immune pathway interactions, laying groundwork for future development of targeted anti-parasitic strategies in aquaculture systems.