Purpose <p>Toxoplasmosis is a worldwide widespread parasitic infection; it affects about 30% of the global population, either through acute toxoplasmosis or its sequels. Even though the male reproductive system is not the primary target for <i>Toxoplasma gondii</i> (<i>T. gondii</i>), studies have inquired into the possibility of presenting repercussions in this system directly or indirectly due to toxoplasmosis. Therefore, this systematic literature review aims to summarize the available evidence on the effects of infection caused by <i>T. gondii</i> on the male reproductive tract.</p> Methods <p>We searched PubMed, Scopus, LILACS and Google Scholar until June 2024 to identify studies of T. gondii and the human male reproductive system. Finally, we analyzed 24 papers published between 1986 and 2024. The Joanna Briggs Institute (JBI) Critical Appraisal Checklist was used to assess the potential risk of bias and the quality of the results.</p> Results <p>Infertility is a multicausal issue, including various stages in which the infection caused by <i>T. gondii</i> could interfere, but the mechanisms are not fully understood yet. Studies in animals, particularly rats, have shown the harmful effects of the parasite on sperm performance and endocrine function. In the same way, sexual transmission of <i>T. gondii</i> has been extensively studied in animals, with the parasite detected in the semen of various species. In humans, this transmission route remains theoretical due to study limitations. However, discrepancies in findings call for further research to understand the mechanisms and make the <i>T. gondii</i>’s infection impact on the male reproductive system a topic of growing interest.</p> Conclusion <p>Acute and chronic infection by <i>T. gondii</i> in the male reproductive system is a topic of growing interest due to its possible implications for reproductive health.</p>