<p>This study aimed to identify the clinical symptoms and indicators that might affect brain activity in patients with amnestic mild cognitive impairment. Thirty-one patients with amnestic mild cognitive impairment were recruited between March 2022 and June 2023. Functional magnetic resonance imaging, neuropsychological tests, and blood tests were also performed. Brain activation was analyzed using voxel- and region-of-interest-based methods. The correlation between the functional magnetic resonance imaging results and the acquired clinical indicators was determined using Pearson’s correlation analysis. In the face-name association memory task, activation of the hippocampus and parahippocampal gyrus was found to be negatively correlated with the Short Form of the Geriatric Depression Scale score. Precentral gyrus and precuneus activation positively correlated with the percentage of correct answers in the one-back task. In the working memory N-back task, activation of the superior parietal lobule was positively correlated with the percentage of correct answers in the zero-back task. Age and activation in the hippocampus and parahippocampal gyrus are positively correlated. Depression, age, and sex significantly affected brain activity. Activation of the precentral gyrus, superior parietal lobule, and precuneus influences N-back task competency. Overall, this study is the first to analyze the correlation between brain function and clinical indicators of amnestic mild cognitive impairment.</p>