<p>Recent research in biomaterials aims to promote a distinct tissue response to create truly biocompatible material. Many modern biomaterials have been developed with different surface energy, size, surface topography, elasticity, etc., to fulfill biocompatibility because the complex process of tissue formation and organization in human is mainly driven by the interaction of cells with their environment. Therefore, it is necessary to understand and control the surface characteristics of biomaterials to optimize their performance in biological environments. In this work, the surface topography of Hydroxyapatite (HAp)-coated polyamide substrate using Pulsed Laser Deposition (PLD) was studied. The topography of the HAp layer produced by PLD was evaluated as a function of substrate temperature, laser pulse energy, and pulse duration because these parameters play an important role in producing a quality layer. The surface topography was evaluated using Scanning Electron Microscope (SEM) and Atomic Force Microscope (AFM), the results revealed that the layer produced at different parameters had a smooth apatite coating with some micro- and nanoparticles scattered over the surface, along with some pits and pillar-like structures was also observed. The RMS roughness and thickness of HAp layer decreased, when there was an increase in substrate temperature at the same time laser pulse energy and pulse duration increased, the RMS roughness and thickness of HAp layer also increased. The results suggest that the surface topography of a HAp layer obtained in this work will have a positive effect on bone bonding and facilitate cellular growth and differentiation.</p> Graphical Abstract <p></p>