<p>The directional conduction cooling affects the microstructural and tribological features in laser powder bed fusion (LPBF). In this work, annealing treatment was applied on a LPBF-processed Ti-6Al-4&#xa0;V (Ti-6-4) alloy to reduce flaws and enhance tribological characteristics. Wet linear reciprocating sliding wear test throughout an immense range of normal loads (5–30 N) was employed in the presence of synthetic physiological media (simulated body fluid) at human biological temperature on lateral surface to investigate their comprehensive wear behavior. The universal tribometer was employed to evaluate wear parameters such as wear rate, volume, depth and coefficient of friction. Morphological and mechanical characteristics were executed and correlated with wear and friction. Wear track width deviations were analyzed using scanning electron microscope (SEM), revealing irregular shape variations. Energy-dispersive spectroscopy (EDS) and worn surface examination supported wear and friction findings. The findings suggest that high-density Ti₃Al precipitates were produced, and the partial decomposition of α' into α and β took place throughout the annealing treatments. The worn morphologies and wear processes depended considerably on the microstructural features and applied stresses. The HT700 sample had a smoother wear surface and lower COF over a wide range of loads due to the formation of protective tribo-oxide layer. Heat treatment of Ti-6-4 alloy resulted in significant improvements in hardness, elongation (EL) and yield strength (YS), with values increasing from 400 ± 5 to 451 ± 25 VHN, 4.64 ± 0.9 to 6.35 ± 0.2 % and 978 ± 11 to 1016 ± 8&#xa0;MPa, respectively. The heat-treated sample showed the better wear behavior and offering potential applications in biomedical implantation.</p>