Efficient in vitro regeneration and phytochemical characterization of medicinal plant Barleria prionitis L.
摘要
An efficient micropropagation method based on axillary shoot bud proliferation was developed for Barleria prionitis, an important Indian woody medicinal plant. Highest (100%) bud break in nodal explants was obtained on Woody Plant Medium (WPM) with 3.0 mg L−1 6-benzyldenine (BA), 0.5 mg L−1 thidiazuron (TDZ), 1.0 mg L−1 indoleacetic acid (IAA) and 500.0 mg L−1 activated charcoal (AC). Shoot culture establishment on liquid Murashige and Skoog (MS) medium with 2.0 mg L−1 BA and 0.5 mg L−1 2,3,5-triiodobenzoic acid (TIBA) followed by subsequent multiplication on solid MS medium with silver nitrate (7.0 mg L−1) increased shoot number (3.4-fold), length (2.5-fold) and leaves/shoot (3.6-fold). Highest per cent rooting (76.6%) was achieved on WPM medium with 2.0 mg L−1 indolebutyric acid (IBA), 0.5 mg L−1 IAA and 0.5 mg L−1 gibberellic acid (GA3). Plantlets survived (85%) upon hardening and acclimatization. Strong antioxidant activity of shoot extracts was demonstrated by FRAP (255.75 ± 0.01 mg AAE g−1), DPPH (IC50 = 12.37 ± 0.22 mg mL−1) and ABTS (IC50 = 2.77 ± 0.09 µg mL−1) assays. High phenolics concentration was established by TPC (27.76 mg of GAE g−1), TFC (31.56 mg of RE g−1) values and HPLC analysis revealed maximum accumulation of gallic acid (83.98 μg g−1) followed by p-Coumaric acid (76.15 μg g−1). Shanzhiside Methyl Ester, an active metabolite of B. prionitis, concentration was 902.10 μg g−1. In conclusion, the micropropagation techniques applied were effective in obtaining clones of B. prionitis, and the higher phytoconstituent content in the shoot cultures highlights their strong potential as an alternative source for phytochemical extraction.