Scholarlink

<p>Efficient and reproducible regeneration procedures are important for the establishment of reliable <i>in vitro</i> systems in medicinal plants. Here, a central composite design (CCD) consisting of 30 treatments was employed for modelling and optimisation of combined influences of kinetin (KI), 6-benzylaminopurine (BAP), indole-3-acetic acid (IAA) and maltose treatments on direct shoot regeneration from immature leaf explants of <i>Enicostemma littorale Blume</i>. Two responses were evaluated, which were the shoot regeneration frequency (Y1%) and the mean shoots per explant (Y2). Across the design space Y1 varied from 58 to 95% while Y2 varied from 6.0 to 30.0. Quadratic models were significant for both responses (Y1: F = 5.67, p = 0.0009; Y2: F = 5.91, <i>p</i> = 0.0008), with R<sup>2</sup> (Y1) and R<sup>2</sup> (Y2) were 0.84 and 0.85, respectively. Significant quadratic terms (<i>p</i> &lt; 0.05) indicated the existence of pronounced curvature and mid-range optima and most linear and interaction terms were found not to be influential at the ranges tested. Box-Cox and residual diagnostics showed no need for transformation (λ ~ 1) and hence, both responses were analyzed on the original scale. Numerical optimisation showed an optimum value of 4.02&#xa0;µM KI, 6.05&#xa0;µM BAP, 4.01&#xa0;µM IAA and 25.10&#xa0;g L⁻<sup>1</sup> maltose with predicted responses of 92.0% (Y1) and 8.40 (Y2). Experimental validation results were obtained as 93.20 ± 2.85% (Y1) and 8.70 ± 0.35 (Y2), which supports the practical predictability of the model. The optimized CCD-based protocol is a reproducible protocol for micropropagation and future <i>in vitro</i> applications, including secondary metabolites, in <i>E. littorale.</i></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Response surface optimization and predictive modeling of shoot regeneration in Enicostemma littorale Blume using central composite design

  • 期刊论文

  • 发表日期：

    2026年6月22日

查看全文
  • Chandran Sureshpandian,
  • G. Mahendraperumal,
  • N. Nirmal Kumar

摘要

Efficient and reproducible regeneration procedures are important for the establishment of reliable in vitro systems in medicinal plants. Here, a central composite design (CCD) consisting of 30 treatments was employed for modelling and optimisation of combined influences of kinetin (KI), 6-benzylaminopurine (BAP), indole-3-acetic acid (IAA) and maltose treatments on direct shoot regeneration from immature leaf explants of Enicostemma littorale Blume. Two responses were evaluated, which were the shoot regeneration frequency (Y1%) and the mean shoots per explant (Y2). Across the design space Y1 varied from 58 to 95% while Y2 varied from 6.0 to 30.0. Quadratic models were significant for both responses (Y1: F = 5.67, p = 0.0009; Y2: F = 5.91, p = 0.0008), with R2 (Y1) and R2 (Y2) were 0.84 and 0.85, respectively. Significant quadratic terms (p < 0.05) indicated the existence of pronounced curvature and mid-range optima and most linear and interaction terms were found not to be influential at the ranges tested. Box-Cox and residual diagnostics showed no need for transformation (λ ~ 1) and hence, both responses were analyzed on the original scale. Numerical optimisation showed an optimum value of 4.02 µM KI, 6.05 µM BAP, 4.01 µM IAA and 25.10 g L⁻1 maltose with predicted responses of 92.0% (Y1) and 8.40 (Y2). Experimental validation results were obtained as 93.20 ± 2.85% (Y1) and 8.70 ± 0.35 (Y2), which supports the practical predictability of the model. The optimized CCD-based protocol is a reproducible protocol for micropropagation and future in vitro applications, including secondary metabolites, in E. littorale.