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<p>A number of factors that have been reported to affect transformation/regeneration rate in&#xa0;plants were studied in multiple eucalypt genotypes. These include surfactant, acetosyringone, fipexide, basal media, activated charcoal, ascorbic acid, lipoic acid, proline, cytokinins, sugars, spectinomycin selection, Agrobacterium strains and concentrations, forchlorfenuron, PVP, silver nitrate, and explant heating prior to cocultivation. Heating, use of acetosyringone, basal medium, cytokinin type, carbon source, and <i>Agrobacterium</i> strain had substantial impacts—whereas the other factors studied had negligible or a negative impact. In search of a model genotype for producing transgenic shoots,&#xa0;53 diverse genotypes that included <i>E. grandis, E. grandis</i> x <i>E. urophylla</i>, <i>E</i>. <i>urophylla</i> x <i>E. grandis,</i> and <i>E. dunnii,</i> were screened&#xa0;using a highly infective gall-causing Agrobacterium strain with T-DNA genes isolated from strain C58. One <i>E. grandis</i> x <i>E. urophylla</i> genotype was identified, Egu45-1, that showed a regeneration rate of transgenic shoots of 4% to 8% (frequency of explants that produced one or more transgenic shoots). This genotype and the associated transformation protocol will be made available to any interested party without restriction.</p>

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Transformation system optimization and introduction of a new model Eucalyptus grandis x urophylla genotype for transgenic studies

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月29日

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  • Cathleen Ma,
  • Ekaterina Peremyslova,
  • Parker Wheeler,
  • Greg S. Goralogia,
  • Steven H. Strauss

摘要

A number of factors that have been reported to affect transformation/regeneration rate in plants were studied in multiple eucalypt genotypes. These include surfactant, acetosyringone, fipexide, basal media, activated charcoal, ascorbic acid, lipoic acid, proline, cytokinins, sugars, spectinomycin selection, Agrobacterium strains and concentrations, forchlorfenuron, PVP, silver nitrate, and explant heating prior to cocultivation. Heating, use of acetosyringone, basal medium, cytokinin type, carbon source, and Agrobacterium strain had substantial impacts—whereas the other factors studied had negligible or a negative impact. In search of a model genotype for producing transgenic shoots, 53 diverse genotypes that included E. grandis, E. grandis x E. urophylla, E. urophylla x E. grandis, and E. dunnii, were screened using a highly infective gall-causing Agrobacterium strain with T-DNA genes isolated from strain C58. One E. grandis x E. urophylla genotype was identified, Egu45-1, that showed a regeneration rate of transgenic shoots of 4% to 8% (frequency of explants that produced one or more transgenic shoots). This genotype and the associated transformation protocol will be made available to any interested party without restriction.