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<p><i>Peucedanum praeruptorum</i> Dunn (<i>P. praeruptorum</i>), a perennial medicinal plant within the <i>Umbelliferae</i> family, is a major traditional Chinese herb. However, its quality has progressively declined during cultivation, and the absence of a genetic transformation system has posed a significant constraint on germplasm innovation and genetic improvement. In this study, a tissue culture regeneration system was established using stem segments as explants. The optimal medium was MS supplemented with 0.5&#xa0;mg·L<sup>−1</sup> NAA and 2.0&#xa0;mg·L<sup>−1</sup> 6-BA, achieving a regeneration rate of 70% (based on ten independent experiments, n = 10–15 per experiment). Based on this established regeneration system, a genetic transformation protocol was developed using <i>Agrobacterium tumefaciens</i> strain EHA105 harboring the pCAMBIA1300 vector carrying the <i>GFP</i> reporter gene. The vigorously grown callus was infected with <i>Agrobacterium</i> suspension (OD<sub>600</sub> = 0.8) by shaking at 200&#xa0;rpm for 30&#xa0;min. Following co-cultivation and selection, <i>GFP</i> expression were monitored using a plant dynamic detection system. Strong fluorescent signals were detected in vigorously proliferating callus cells. After screening and differentiation, the callus developed into seedlings, where strong fluorescent signals was observed. This indicates that <i>GFP</i> is expressed in the regenerated seedlings of <i>P. praeruptorum</i>. This study provides a foundational method for genetic improvement of <i>P. praeruptorum</i> and may be applicable to related medicinal plants after further optimization.</p>

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Development of a genetic transformation system for Peucedanum praeruptorum Dunn

  • 期刊论文

  • 发表日期：

    2026年4月1日

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  • Peipei Wei,
  • Jun Dai,
  • Xingen Zhang,
  • Maosuo Wang,
  • Shenghua Wang,
  • Qian Ruan,
  • Huifang Chu,
  • Binbin Du

摘要

Peucedanum praeruptorum Dunn (P. praeruptorum), a perennial medicinal plant within the Umbelliferae family, is a major traditional Chinese herb. However, its quality has progressively declined during cultivation, and the absence of a genetic transformation system has posed a significant constraint on germplasm innovation and genetic improvement. In this study, a tissue culture regeneration system was established using stem segments as explants. The optimal medium was MS supplemented with 0.5 mg·L−1 NAA and 2.0 mg·L−1 6-BA, achieving a regeneration rate of 70% (based on ten independent experiments, n = 10–15 per experiment). Based on this established regeneration system, a genetic transformation protocol was developed using Agrobacterium tumefaciens strain EHA105 harboring the pCAMBIA1300 vector carrying the GFP reporter gene. The vigorously grown callus was infected with Agrobacterium suspension (OD600 = 0.8) by shaking at 200 rpm for 30 min. Following co-cultivation and selection, GFP expression were monitored using a plant dynamic detection system. Strong fluorescent signals were detected in vigorously proliferating callus cells. After screening and differentiation, the callus developed into seedlings, where strong fluorescent signals was observed. This indicates that GFP is expressed in the regenerated seedlings of P. praeruptorum. This study provides a foundational method for genetic improvement of P. praeruptorum and may be applicable to related medicinal plants after further optimization.