Scholarlink

<p>Genetically modified hairy roots (HR) of <i>Peganum harmala</i>, developed through inoculation with the A4 strain of <i>Rhizobium rhizogenes</i> (<i>Agrobacterium rhizogenes)</i> and cultivated <i>in vitro</i>, demonstrated a significant capacity for high biomass production, thereby offering potential for enhanced indole alkaloid levels. The successful transfer of the <i>RolB</i> gene from the A4 strain of <i>R. rhizogenes</i> to the L<sub>2</sub> and L<sub>6</sub> hairy root lines of <i>P. harmala</i> was confirmed <i>via</i> polymerase chain reaction (PCR) analysis. To further improve both biomass growth and the biosynthesis of β-carboline alkaloids, three <i>Pseudomonas</i> strains (P64, P66, and C7R12) were utilized as biotic elicitors, applied over three different contact durations with two selected hairy root lines (L<sub>2</sub> and L<sub>6</sub>). The findings indicated that the L<sub>2</sub> line achieved the highest biomass after a 3 d elicitation period with the P66 strain, resulting in an increase of approximately 0.2 g of dry weight compared to the control. Upon extraction and quantification of the indole alkaloid harmine, using gas chromatography coupled with mass spectrometry (GC–MS), the highest harmine concentration was observed in the L<sub>2</sub> line treated with the P66 strain for 5 d, yielding 22.50 ± 0.74 mg&#xa0;g⁻<sup>1</sup> dry weight, which represented a 75.5% increase over the control.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Enhancing biomass yield and harmine accumulation in Peganum harmala L. hairy roots through biotic elicitation by Pseudomonas sp.

  • 期刊论文

  • 发表日期：

    2026年5月26日

查看全文
  • Nouara Bakiri,
  • Eric Gontier,
  • Abdelkader Morsli,
  • Roukia Benyammi,
  • Lakhdar Khelifi

摘要

Genetically modified hairy roots (HR) of Peganum harmala, developed through inoculation with the A4 strain of Rhizobium rhizogenes (Agrobacterium rhizogenes) and cultivated in vitro, demonstrated a significant capacity for high biomass production, thereby offering potential for enhanced indole alkaloid levels. The successful transfer of the RolB gene from the A4 strain of R. rhizogenes to the L2 and L6 hairy root lines of P. harmala was confirmed via polymerase chain reaction (PCR) analysis. To further improve both biomass growth and the biosynthesis of β-carboline alkaloids, three Pseudomonas strains (P64, P66, and C7R12) were utilized as biotic elicitors, applied over three different contact durations with two selected hairy root lines (L2 and L6). The findings indicated that the L2 line achieved the highest biomass after a 3 d elicitation period with the P66 strain, resulting in an increase of approximately 0.2 g of dry weight compared to the control. Upon extraction and quantification of the indole alkaloid harmine, using gas chromatography coupled with mass spectrometry (GC–MS), the highest harmine concentration was observed in the L2 line treated with the P66 strain for 5 d, yielding 22.50 ± 0.74 mg g⁻1 dry weight, which represented a 75.5% increase over the control.