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<p><i>Barbacenia purpurea</i> Hook. is a plant from the Velloziaceae family that tolerates extreme dryness (such as vegetative desiccation tolerance), a trait with high ecological and translational importance. However, its slow growth and limited distribution restrict its availability for research. In this study, an <i>in vitro</i> propagation system for <i>B. purpurea</i> was developed, incorporating shoot multiplication, rooting, and acclimation. Seedlings were cultured on half-strength Murashige and Skoog medium, and cytokinin-auxin combinations based on 6-benzylaminopurine (BAP) were tested for their effects on shoot proliferation. Treatments with BAP and α-naphthaleneacetic acid (NAA) increased the number of shoots but also caused callus formation and morphological variability, whereas hormone-free media promoted uniform shoot elongation. Rooting occurred naturally in media containing activated charcoal, without added exogenous auxins. The plants' acclimation to the substrate resulted in a high survival rate (88%), and they reached maturity within 11 to 15&#xa0;months (mo), considerably faster than other desiccation-tolerant species, allowing for rapid multiplication of plant material. Acclimated plants also retained vegetative desiccation tolerance, demonstrating that <i>in vitro</i>-propagated <i>B. purpurea</i> can be used for physiological studies without the need for multi-year plant maturation. This protocol addresses the limitations caused by the slow growth of desiccation-tolerant <i>Barbacenia</i> species and provides a reliable system for producing experimental material to support physiological, molecular, and developmental research. It enables faster and more abundant production compared to conventional propagation methods.</p>

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Development of an in vitro propagation system for the desiccation-tolerant Barbacenia purpurea

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年4月18日

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  • Pablo Silva-Villatoro,
  • Gerardo Alejo-Jacuinde,
  • Shumayla,
  • Luis Herrera-Estrella

摘要

Barbacenia purpurea Hook. is a plant from the Velloziaceae family that tolerates extreme dryness (such as vegetative desiccation tolerance), a trait with high ecological and translational importance. However, its slow growth and limited distribution restrict its availability for research. In this study, an in vitro propagation system for B. purpurea was developed, incorporating shoot multiplication, rooting, and acclimation. Seedlings were cultured on half-strength Murashige and Skoog medium, and cytokinin-auxin combinations based on 6-benzylaminopurine (BAP) were tested for their effects on shoot proliferation. Treatments with BAP and α-naphthaleneacetic acid (NAA) increased the number of shoots but also caused callus formation and morphological variability, whereas hormone-free media promoted uniform shoot elongation. Rooting occurred naturally in media containing activated charcoal, without added exogenous auxins. The plants' acclimation to the substrate resulted in a high survival rate (88%), and they reached maturity within 11 to 15 months (mo), considerably faster than other desiccation-tolerant species, allowing for rapid multiplication of plant material. Acclimated plants also retained vegetative desiccation tolerance, demonstrating that in vitro-propagated B. purpurea can be used for physiological studies without the need for multi-year plant maturation. This protocol addresses the limitations caused by the slow growth of desiccation-tolerant Barbacenia species and provides a reliable system for producing experimental material to support physiological, molecular, and developmental research. It enables faster and more abundant production compared to conventional propagation methods.