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<p><i>Reineckia carnea</i> (Andrews) Kunth., a traditional ethnomedicinal plant of the Miao community in China with notable ornamental value, faces propagation challenges due to its prolonged sexual reproduction cycle and inefficient vegetative reproduction. This study aimed to establish an efficient <i>in vitro</i> propagation system for <i>R. carnea</i>. Wild superior individuals were selected, and newly sprouted young buds were used as explants. Single-factor experiments, full factorial combinations, and orthogonal design [L<sub>9</sub>(3<sup>4</sup>)] were conducted to evaluate the effects of phytohormones and their concentrations on rhizome enlargement, shoot cluster induction, proliferation, and plant regeneration were systematically evaluated. Single-factor tests demonstrated that cytokinins significantly outperformed auxins in rhizome swelling and adventitious shoot induction, while auxins were more effective in root initiation. Full factorial combinations (6-BA combined with NAA, 6-BA combined with 2,4-D) induced rhizome swelling and basal shoot formation but yielded suboptimal proliferation rates. Subsequent orthogonal experiments identified the optimal hormone combination for basal shoot proliferation as 2.5&#xa0;mg·L<sup>−1</sup> 6-BA with 1.0&#xa0;mg·L<sup>−1</sup> NAA and 0.5&#xa0;mg·L<sup>−1</sup> 2,4-D, achieving a proliferation of basal stem cluster buds exceeding 8.0. Rooting was optimized on 1/2 MS supplemented with 1.5&#xa0;mg·L<sup>−1</sup> IBA resulting in 100% rooting after 60 d. All acclimatized plantlets survived post-transplantation. A rapid propagation system for <i>R. carnea</i> was successfully established <i>via</i> direct organogenesis from clustered basal shoots. This system not only ensured high proliferation efficiency but also enhanced plantlet quality, providing a foundation for commercial production and future genetic transformation studies.</p>

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Establishment of an artificial rapid propagation system for Reineckia carnea (Andrews) Kunth. via induction of clustered basal shoots

  • 期刊论文

  • 发表日期：

    2026年4月2日

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  • Li-Qing Cheng,
  • Xiao-Qing Ge,
  • Heng-Yu Huang

摘要

Reineckia carnea (Andrews) Kunth., a traditional ethnomedicinal plant of the Miao community in China with notable ornamental value, faces propagation challenges due to its prolonged sexual reproduction cycle and inefficient vegetative reproduction. This study aimed to establish an efficient in vitro propagation system for R. carnea. Wild superior individuals were selected, and newly sprouted young buds were used as explants. Single-factor experiments, full factorial combinations, and orthogonal design [L9(34)] were conducted to evaluate the effects of phytohormones and their concentrations on rhizome enlargement, shoot cluster induction, proliferation, and plant regeneration were systematically evaluated. Single-factor tests demonstrated that cytokinins significantly outperformed auxins in rhizome swelling and adventitious shoot induction, while auxins were more effective in root initiation. Full factorial combinations (6-BA combined with NAA, 6-BA combined with 2,4-D) induced rhizome swelling and basal shoot formation but yielded suboptimal proliferation rates. Subsequent orthogonal experiments identified the optimal hormone combination for basal shoot proliferation as 2.5 mg·L−1 6-BA with 1.0 mg·L−1 NAA and 0.5 mg·L−1 2,4-D, achieving a proliferation of basal stem cluster buds exceeding 8.0. Rooting was optimized on 1/2 MS supplemented with 1.5 mg·L−1 IBA resulting in 100% rooting after 60 d. All acclimatized plantlets survived post-transplantation. A rapid propagation system for R. carnea was successfully established via direct organogenesis from clustered basal shoots. This system not only ensured high proliferation efficiency but also enhanced plantlet quality, providing a foundation for commercial production and future genetic transformation studies.